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pT7gRNA-ccdb

Plasmid

#140864

Purpose

Backbone for making sgRNAs.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Use

Gateway destination vector

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-639 (attR4-attR2)

Plasmid

#161880

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Insect

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-HypaCas9-D1135E

Plasmid

#154930

Purpose

aTc inducible HypaCas9 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

HypaCas9-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N692A/M694A/Q695A/H698A/D1135E

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-D1135E

Plasmid

#154924

Purpose

aTc inducible Cas9HF1 with D1135E mutation

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

Cas9HF1-D1135E

Use

CRISPR and Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A/D1135E and stop codon on …

Promoter

pTet

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0548

Plasmid

#162299

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEE22

Plasmid

#161787

Purpose

Hi-SC2 reporter delivery plasmid

Depositor

François-Xavier Barre

Article

Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

Insert

Hi-SC2 reporter

Use

Conjugative plasmid to deliver hi-sc2 reporter

Mutation

Himar site included MmeI site, tandem loxP sites,…

Promoter

n/a

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0545

Plasmid

#162296

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0542

Plasmid

#162293

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-GST-[thrombin cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Integrase triple controller

Plasmid

#126540

Purpose

Pben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+

Depositor

Jerome Bonnet

Article

Zuniga et al Nat Commun. 2020 Sep 21;11(1):4758. doi:

Insert

BenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1

Expression

Bacterial

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1

Plasmid

#145369

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Tags

His and eGFP

Expression

Bacterial

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA(∆50-56)

Plasmid

#134684

Purpose

Expresses His6-AmmA(∆50-56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(del50-56)

Tags

His6

Expression

Bacterial

Mutation

deletion of amino acids 50-56

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA

Plasmid

#134682

Purpose

Expresses His6-AmmA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-P3P1r

Plasmid

#141014

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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CSIV-DEST

Plasmid

#141053

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R4

Plasmid

#141054

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pHygTK

Plasmid

#141059

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HygTK

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R5R4

Plasmid

#141055

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pPuroTK

Plasmid

#141060

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-deltaKRMR-3xFLAG

Plasmid

#130676

Purpose

Expresses human SMCHD1 deltaKRMR with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 deltaKRMR (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

K1987_R1990del

Promoter

CMV

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pFd022.R2.RFP

Plasmid

#137971

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2.RFP

Plasmid

#137972

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2.RFP

Plasmid

#137973

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd031.RFP

Plasmid

#137974

Purpose

Expresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus MIT9211 ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1226

Plasmid

#138593

Purpose

Expresses NatL2-R156A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Arginine 156 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1222

Plasmid

#138592

Purpose

Expresses NatL2-K418A in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Mutation

changed Lysine 418 to Alanine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pMM587

Plasmid

#133593

Purpose

Part Plasmid for dCas9, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMM586

Plasmid

#133592

Purpose

Part Plasmid for dCas9, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_019

Plasmid

#123800

Purpose

Encodes dsaCAS9 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SadCas9

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM765

Plasmid

#133602

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM756

Plasmid

#133600

Purpose

Part Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG25

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM729

Plasmid

#133598

Purpose

Part Plasmid for Mxi1-dCas9, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Mxi1-dCas9

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM767

Plasmid

#133604

Purpose

Part Plasmid for dCas9 NLS, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM585

Plasmid

#133591

Purpose

Part Plasmid for dCas9, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM766

Plasmid

#133603

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM768

Plasmid

#133605

Purpose

Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM769

Plasmid

#133606

Purpose

Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM755

Plasmid

#133599

Purpose

Part Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG11

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM764

Plasmid

#133601

Purpose

Part Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgTEF1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3_028

Plasmid

#124054

Purpose

Encodes c120 sg RNA guide cleaved by ribozyme

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RIbozyme (C120 sg RNA)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK8b_006

Plasmid

#123894

Purpose

Encodes the LoxP recognition site as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LoxP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK8b_008

Plasmid

#123895

Purpose

Encodes the 3' 500 bp cRosa26 locus homology arm as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LA_cROSA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK7_006

Plasmid

#123874

Purpose

Encodes the FRT cassette as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

FRT

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK6_006

Plasmid

#123860

Purpose

Encodes a spacer cassette as a type 6 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_006

Plasmid

#123820

Purpose

Encodes ER Targeting Sequence (N Terminal) as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

KDEL

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_008

Plasmid

#123822

Purpose

Encodes Golgi Targeting Sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GTS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_012

Plasmid

#123825

Purpose

Encodes mScarlet as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_015

Plasmid

#123828

Purpose

Encodes msfGFP as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

msfGFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_023

Plasmid

#123835

Purpose

Encodes Gibberellin Insensitive as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GAI

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_018

Plasmid

#123799

Purpose

Encodes dspCAS9 as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SpdCas9

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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