We narrowed to 2,267 results for: D10
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Plasmid#161815PurposeExpresses ABE8eWQ in mammalian cellsDepositorInsertbpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
UseCRISPRExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ151
Plasmid#69302PurposeGateway entry vector with pcoCas9D10ADepositorInsertpcoCas9D10A (Plant codon-optimized)
UseCRISPRTags2XFLAGMutationD10A mutation; NLS fusionAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRMutationD10AAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRMutationD10A mutation; NLS fusionAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ159
Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGMutationD10A mutation; NLS fusionAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVF28
Plasmid#229469PurposeExpresses RssB with D104A mutationDepositorInsertRssB (D104A)
ExpressionBacterialMutationD104APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#229471PurposeExpresses RssB with D104N mutation with His-tagDepositorInsertRssB (D104N)
Tags10xHisExpressionBacterialMutationD104NPromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS23
Plasmid#229470PurposeExpresses RssB with D104A mutation with His-tagDepositorInsertRssB (D104A)
Tags10xHisExpressionBacterialMutationD104APromoterT7Available SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265N1
Plasmid#173998PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-UNG
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationD10AAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265O1
Plasmid#173999PurposeGateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editingDepositorInsertrAPOBEC1-zCas9(D10A)-rXRCC1
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationD10AAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0202
Plasmid#91003PurposeModule A, Promoter: AtUbi10, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRMutationD10APromoterAtUbi10Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT31
Plasmid#223403PurposeT-DNA vector for SpCas9-D10A based A-to-G base editing for dicot plants; NGG PAM; SpCas9D10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpCas9-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT39
Plasmid#223411PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.DepositorInsert2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT42
Plasmid#223414PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT38
Plasmid#223410PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants select.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT37
Plasmid#223409PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.DepositorInsertAtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT40
Plasmid#223412PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3; Hygromycin for plants selection.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNW3
Plasmid#53372PurposeCsy4 and Cas9 D10A nickase expression plasmidDepositorInsertCsy4-T2A-Cas9n (D10A)
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1210
Plasmid#91033PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_D10A nickase, Terminator: HSPDepositorInsertTaCas9_D10A nickase
UseCRISPRMutationD10APromoterZmUbiAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0201
Plasmid#91002PurposeModule A, Promoter: 35S, Gene: AtCas9_D10A nickase, Terminator: HSPDepositorInsertAtCas9_D10A nickase
UseCRISPRMutationD10APromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only