We narrowed to 7,550 results for: PAC

1. pAct-dCBEevoCDA1

   Plasmid

   #195515

   Purpose

   Ubiquitous expression of dCBEevoCDA1 in Drosophila

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   dCBE-evoCDA1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. H9 F42A pAcGP67a

   Plasmid

   #41810

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   H9 Super-2 (IL2 Human)
   

   Use

   Tags

   8x His

   Expression

   Insect

   Mutation

   Phenylalanine 42 to Alanine

   Promoter

   Polyhedron

   Available since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pGEM-HE-h_bPAC_cmyc

   Plasmid

   #28134

   Purpose

   optogenetic tool for light modulation of cellular cAMP

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   humanized photoactivated adenylyl cyclase
   

   Use

   In vitro transcription

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-HypaCas9

   Plasmid

   #190604

   Purpose

   PX458 (Plasmid #48138) with the N692A, M694A, Q695A, and H698A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Takahashi et al iScience. 2022 Nov 17;25(12):105619. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC059-HisMS2_PLP_NucPhos_pac

   Plasmid

   #155039

   Purpose

   Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

   Depositor

   Paul Freemont

   Article

   Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

   Inserts

   Maturation Protein
   Coat Protein Dimer
   Nucleocapsid Phosphoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Remove TypeIIs Restriction Sites

   Promoter

   T7

   Available since

   Sept. 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-PACT

   Plasmid

   #15667

   Depositor

   Dong-Yan Jin

   Article

   Kok et al J Biol Chem. 2007 Jun 15. 282(24):17649-

   Insert

   PACT (PRKRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pAcGP67A-TfR

   Plasmid

   #12130

   Depositor

   Pamela Bjorkman

   Article

   Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

   Insert

   transferrin receptor (TFRC Human)
   

   Use

   Tags

   6X-His tag, Factor Xa cleavage site, and leader p…

   Expression

   Insect

   Mutation

   The portion of the human TfR gene encoding residu…

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc-C Med12His

    Plasmid

    #49240

    Purpose

    expresses human Med12 with His tag in insect cells

    Depositor

    Dylan Taatjes

    Article

    Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

    Insert

    Med12 (MED12 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEvol-pAcFRS.2.t1

    Plasmid

    #73544

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAcFRS.2.t1
    pAcFRS.2.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-HypaCas9

    Plasmid

    #108302

    Purpose

    pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-PACT

    Plasmid

    #50352

    Purpose

    bacterial expression of PACT

    Depositor

    Kumiko Ui-Tei

    Article

    Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

    Insert

    PACT (PRKRA Human)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc V5 dDispatched WT HA

    Plasmid

    #126405

    Purpose

    Expression of V5 dDispatched WT HA in Drosophila cells lines.

    Depositor

    Stacey Ogden

    Article

    Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

    Insert

    d-Dispatched1 (disp Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Epac-S-H74

    Plasmid

    #170338

    Purpose

    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:

    Insert

    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAcGFP1-N1-RvCAHS3-GS

    Plasmid

    #192460

    Purpose

    Expression of RvCAHS3-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Epac-SH225

    Plasmid

    #191449

    Purpose

    expression of cAMP sensor in yeast

    Depositor

    Kees Jalink

    Insert

    mT2Del-EPACdDEPCD-tdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcBac1-Mb-sfGFP WT

    Plasmid

    #197569

    Purpose

    Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP WT
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SPACE-NG (pRZ5150)

    Plasmid

    #140244

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

    Promoter

    CMV

    Available since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only