We narrowed to 17,815 results for: IGH@

Showing: 6951 - 7000 of 17815 results

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dKatushka-pBAD

Plasmid

#54775

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635

Depositor

Dmitriy Chudakov, Michael Davidson

Article

Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

Type

Empty backbone

Tags

6xHis and dKatushka

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2_166

Plasmid

#199098

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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EcMBP165-cpGFP.PPYF.T203V.pRSET

Plasmid

#33373

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

Improved MBP based Maltose Biosensor

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pAH25-SceI

Plasmid

#129389

Purpose

Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Chloramphenicol acetyltransferase

Expression

Bacterial

Mutation

N/A

Promoter

CAT promoter

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGL2_167

Plasmid

#199099

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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Nano-lantern(ATP1)/pcDNA3

Plasmid

#51992

Purpose

mammalian expression of a Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Expression

Mammalian

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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B2088_BTB-IDR-FRB

Plasmid

#215101

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_K356D

Plasmid

#214312

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

K356D

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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TC1405

Plasmid

#164877

Purpose

BFP-preSTOP-GFP reporter

Depositor

Tian Chi

Article

Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

Insert

BFP-preSTOP-GFP

Expression

Mammalian

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C-Src-7

Plasmid

#55409

Purpose

Localization: Membrane/Vesicles, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

C-Src

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCasper_Pdf-LexA::VP16

Plasmid

#33259

Depositor

Michael Rosbash

Article

Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

Insert

LexA::VP16-SV40 3'UTR (lexA E. coli)

Expression

Insect

Promoter

Pdf

Available Since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only
pMK377 (Hygro-P2A-mCherry2)

Plasmid

#121188

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mCherry2

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - NR

Plasmid

#89625

Purpose

Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Nitrate reductase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNAJB1_DNAJ

Plasmid

#109918

Purpose

Protein expression and purification of DNAJB1_DNAJ

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNAJB1_DNAJ

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHPdesteGFP

Plasmid

#24566

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

eGFP

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH72

Plasmid

#202487

Purpose

Expresses the nanobody VHH72 with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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sfTq2-N1

Plasmid

#117931

Purpose

Superfolder mTurquoise2 (pNM112)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2

Expression

Mammalian

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFG036 + pFG051

Plasmid

#137997

Purpose

Transposon mutagenesis plasmid and the cI repressor expression plasmid

Depositor

Sébastien Rodrigue

Article

Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

Insert

Tn5 tnp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSTK120-mCMV-TK

Plasmid

#22054

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-TK-pA expression cassette

Use

Adenoviral

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
env8 reverse J1/13

Plasmid

#99831

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 reverse J1/13

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 sequence is reversed

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EcMBP165-cpGFP.PPYF.pRSET

Plasmid

#33372

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

MBP based Maltose Biosensor

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available Since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pFPL-Rg

Plasmid

#61654

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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LaM8-AK74

Plasmid

#159594

Purpose

Opto-Nanobody

Depositor

Jose Avalos, Jared Toettcher

Article

Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

Insert

LaM8 with AsLOV2 at AK74

Expression

Bacterial

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP709

Plasmid

#79986

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-9xSeq2-Luc

Plasmid

#42214

Purpose

Luciferase reporter for GI-ZFP2

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

9xSeq2 Binding Sites

Use

Luciferase

Expression

Mammalian

Promoter

9 tandem copies of Seq2 + minimal human CMV promo…

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pLV-EgI-ZeoR

Plasmid

#207178

Purpose

Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-ZeoR

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Ef1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRPaCas9

Plasmid

#111819

Purpose

Expresses Cas9 in T. brucei (2T1) cells

Depositor

David Horn

Article

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Insert

Cas9

Use

CRISPR

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
B2395_CIBN-BTB-IDR-FRB

Plasmid

#215107

Purpose

Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CIBN-BTB-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPLC

Plasmid

#122261

Purpose

pBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- B0015

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Nov 16;7(11):2627-2

Insert

pBRBAD, pBRlasI

Use

Synthetic Biology

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF/myc/ER FLII12Pglu-700uDelta6

Plasmid

#17867

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Lysosomes-20

Plasmid

#58022

Purpose

Localization: Lysosome Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

LAMP1

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pHPdestmCherry

Plasmid

#24567

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

mCherry

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-Myc-TEAD4-Y429H

Plasmid

#33041

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Mutation

Y429H

Available Since

March 28, 2012

Availability

Academic Institutions and Nonprofits only
pRSFDuet1-sgRNA(siteT9)

Plasmid

#154832

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteT9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCri_L706-antiSH2-OptoMB

Plasmid

#159935

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ-dpcoCas9-TV

Plasmid

#158409

Purpose

CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Insert

dpcoCas9-TV(6xTAL-2xVP64)

Use

CRISPR

Expression

Plant

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDICI

Plasmid

#51652

Purpose

E coli genetic modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

CmR

Use

Genetic modification

Promoter

unknown

Available Since

July 7, 2014

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT21.RY

Plasmid

#201006

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

mcherry
sYFP2

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7-SCOUT21.YB

Plasmid

#201008

Purpose

pLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Inserts

sYFP2
TagBFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3-Rabaptin-5

Plasmid

#199173

Purpose

Expresses GFP-Rabaptin-5 fusion in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

Rabaptin-5 (RABEP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains Tyr 149 Ser substitution; insert starts …

Promoter

CMV

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK408 (mAID-Nluc Hygro)

Plasmid

#121198

Purpose

C-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

mAID-Nluc Hygro

Use

CRISPR

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEIAV-SIN6.1 CBLacZ-LucWm1A

Plasmid

#44167

Depositor

John Olsen

Article

Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

Inserts

LacZ
firefly luciferase

Use

Lentiviral and Luciferase

Promoter

CB, CMV enhancer/chicken beta-actin promoter

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-mito-CitroffRH

Plasmid

#158548

Purpose

Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitroffRH

Expression

Mammalian

Promoter

cmv

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G01

Plasmid

#67135

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFPNL1

Plasmid

#61651

Purpose

Gateway vector for generating nucleus-targeted fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
GeNL(Ca2+)_60/pcDNA3

Plasmid

#85207

Purpose

luminescent reporter for calcium signaling

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

GeNL(Ca2+)_60

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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