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Plasmid#110728PurposeMammalian Expression of p16INK4A IRES zsGreenDepositorAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorPromoterZ3EVAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Nopt
Plasmid#231704PurposeMammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE-Plus
Plasmid#214079PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dnDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE-Plus
ExpressionMammalianPromoterCMVAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-OGT_opt
Plasmid#190858PurposeFor mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertO-GlcNAc Transferase (OGT Human)
ExpressionMammalianMutationCodon optimized for expression in human cells.PromoterCMVAvailable sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
ExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
Plasmid#78101PurposeExpresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-CAG-dCasRx-RBM25-IRES-BleoR
Plasmid#221002PurposeStable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx-RBM25-IRES-BleoR
UseCRISPRTagsNLS and NLS-HAExpressionMammalianPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
U6-hGRIN2B-CAG-ps-SpCas9
Plasmid#102851PurposeA single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B S. pyogenes, Human, Synthetic)
UseCRISPRTags3X Flag and NLSExpressionMammalianPromoterU6 promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-iPE-Plus
Plasmid#214062Purposeinducible PE-Plus system for controllable prime editing; this plasmid is used to insert PE-Plus prime editor (PEmax-P2A-hP53DD-IRES-MLH1dn) in one allele of AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE-Plus
ExpressionMammalianPromoterTRE-tightAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS G13D
Plasmid#111851PurposeExpresses activated human KRAS G13D (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS G13D (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialMutationChanged Glycine 13 to Aspartic AcidPromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Target-AID (pRZ762)
Plasmid#131300PurposeCAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
ExpressionMammalianPromoterCAGAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hSNCA-NE
Plasmid#102361PurposeOverexpression of synuclein alpha in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsynuclein alpha (SNCA Human)
TagsNEExpressionMammalianPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-lck-jGCaMP8f
Plasmid#176759PurposeAAV-mediated expression of membrane bound GCaMP8f under the control of GfaABC1D promoterDepositorHas serviceAAV1 and AAV9Insertlck-jGCaMP8f
UseAAVExpressionMammalianPromoterGfaABC1DAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-syn-flex-iGluSnFR4f-NGR-WPRE
Plasmid#234442PurposeAAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.DepositorHas serviceAAV1InsertiGluSnFR4f-NGR
UseAAV and Cre/LoxTagsIgK chain and Myc epi tag-NGR GPI anchorExpressionMammalianPromoterSynapsinAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA-mut
Plasmid#140558PurposeExpresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neuronsDepositorHas serviceAAV9InsertRed fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
UseAAVPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3
Plasmid#172923PurposeFor enriched expression of jGCaMP8f in axons with cytosolic expression of mRuby3DepositorHas serviceAAV1 and AAV9Insertaxon-jGCaMP8f-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain (N terminal on insert…ExpressionMammalianPromoterhSynapsin1Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-emiRFP670
Plasmid#197200PurposeExpresses the protein of emiRFP670 in mammalian cellsDepositorHas serviceAAV1InsertemiRFP670
UseAAVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only