We narrowed to 38,903 results for: LAT;

1. pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)

   Plasmid

   #122067

   Purpose

   Expressing Dhh1-PA

   Depositor

   Daniel Klionsky

   Article

   Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

   Insert

   Dhh1-PA (DHH1 Budding Yeast)
   

   Use

   Tags

   PA

   Expression

   Yeast

   Mutation

   Aspartic acid 195 to alanine; Glutamic acid 196 t…

   Promoter

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ST-SBP-EGFP_puromycin

   Plasmid

   #65270

   Purpose

   ST reporter only ( no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3 Flag BimL (AA)

   Plasmid

   #24240

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   Bim L(AA) (BCL2L11 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   changed Threonine 56 to Alanine changed Serine …

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pSilencer2.1-U6-NL1F35

   Plasmid

   #190830

   Purpose

   For mammalian expression of NL1F35, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   NL1F35 (A Dual-Specificity aptamer targeting OGT and GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3.2/NS4A-2K

   Plasmid

   #120820

   Purpose

   Ectopic expression of ZIKV protein

   Depositor

   Jan Rehwinkel

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   NS4A + 2K ZIKV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3 14 mut B-Myb

   Plasmid

   #25967

   Depositor

   Rob Lewis

   Article

   Johnson et al J Biol Chem. 2002 Feb 8. 277(6):4088-97.

   Insert

   B-Myb (Mybl2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Changed threonines 267, 408, 443, 447 490, 497, 5…

   Promoter

   Available since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

7. ATF4 11: 5’ATF4.uORF2.GFP

   Plasmid

   #21858

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   ATF4 (Atf4 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   upstream ORF2 (uORF) of ATF4 fused to EGFP

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-OSBP

   Plasmid

   #32495

   Depositor

   Alex Toker

   Article

   Nhek et al Mol Biol Cell. 2010 Jul 1;21(13):2327-37

   Insert

   OSBP (OSBP Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3_PKA-CαN (K29,30A)-mVenus

   Plasmid

   #168485

   Purpose

   Mammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.

   Depositor

   Haining Zhong

   Article

   Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

   Insert

   the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

    Plasmid

    #149661

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30(-10AC12), Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T1-Claspin-N

    Plasmid

    #31623

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    aa 1-679 of N-term

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

12. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBSSK p85 alpha (delta SH3)

    Plasmid

    #13430

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta SH3) (PIK3R1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    SH3 domain replaced by flag tag.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pBABE-hygro NM23 H1 WT

    Plasmid

    #11364

    Depositor

    Ronald Kahn

    Article

    Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

    Insert

    NM23 H1 (NME1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pET-30a(+)-Syk-tSH2

    Plasmid

    #111269

    Purpose

    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

    Depositor

    Carol Post

    Article

    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

    Insert

    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSico-shRheb

    Plasmid

    #81087

    Purpose

    conditional expression of an shRNA targeting murine Rheb

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    Rheb shRNA (Rheb Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCLXSN-HA-CYLD

    Plasmid

    #27556

    Depositor

    Shao-Cong Sun

    Article

    Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

    Insert

    CYLD (CYLD Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R156G and G179R

    Promoter

    Available since

    Aug. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

18. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATF4 14: 5’ATF4.uORF1&2AUA.GFP

    Plasmid

    #21861

    Depositor

    David Ron

    Article

    Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

    Insert

    ATF4 (Atf4 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    AUG codons of uORFs1&2 were converted to AUA

    Promoter

    Available since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

    Plasmid

    #149588

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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