We narrowed to 13,845 results for: Cas9

1. MMW3645 - human expression plasmid for SpCas9 Cluster 3

   Plasmid

   #101187

   Purpose

   Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   T657A/G658A/W659A/R661A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MMW3770 - human expression plasmid for SpCas9 Cluster 4

   Plasmid

   #101189

   Purpose

   Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   F491A/M495A/T496A/N497A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MMW2993 - human expression plasmid for SpCas9 Cluster 2

   Plasmid

   #101185

   Purpose

   Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster2(G582A/V583A/E584A/D585A/N588A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   G582A/V583A/E584A/D585A/N588A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

   Plasmid

   #91884

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Gfi1 (Gfi1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

5. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

   Plasmid

   #91877

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Gfi1 (Gfi1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

6. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

   Plasmid

   #91883

   Purpose

   Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   gRNA targeting Gfi1 (Gfi1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

   Plasmid

   #69221

   Purpose

   Expresses R1333K mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1333 is changed to Lysine

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

   Plasmid

   #69222

   Purpose

   Expresses R1333S mutant SpCas9 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Arginine 1333 is changed to Serine

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

   Plasmid

   #102245

   Purpose

   Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

   Depositor

   Jacob Corn

   Insert

   dCas9-VPR-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-VPR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

    Plasmid

    #188767

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

    Plasmid

    #172982

    Purpose

    control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

    Plasmid

    #188768

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

    Plasmid

    #212829

    Purpose

    Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.

    Depositor

    Wolfgang Enard

    Article

    Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

    Insert

    ZIM3-KRAB-dCas9-P2A-mCherry
    

    Use

    Tags

    HA-tag and ZIM3-KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG, TetOn

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid

    #175571

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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