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pGL3-U6-sgRNA_VEGFA-mcherry

Plasmid

#159790

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human VEGFA gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting VEGFA gene (VEGFA Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-tRNAx2

Plasmid

#123641

Purpose

Mammalian expression, Genome editing, backbone

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSLBPres

Plasmid

#82454

Purpose

Encodes synthesized SLBP that is resistant to SMARTpool siRNA

Depositor

Bharat Ramratnam

Article

Li et al J Clin Invest. 2016 Jul 25. pii: 82360.

Insert

SLBP (SLBP Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
U_(pos36GFP)-Cys

Plasmid

#71758

Purpose

U_+36GFP-Cys fusion expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_+36GFP-Cys

Tags

6XHis

Expression

Bacterial

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHL-EF1a-SphcCas9(D10A)-iP-A

Plasmid

#60600

Purpose

Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Inserts

CRISPR Cas9 D10A
puromycin resistance gene

Use

CRISPR

Expression

Mammalian

Mutation

Changed Asp 10 to Ala, Codon usage optimized for …

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-AGO4

Plasmid

#24319

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

AGO4 (AGO4 Human)

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only
HA-AMOT p130 Y242A

Plasmid

#32823

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

HA

Expression

Mammalian

Mutation

E5D, Y242A

Promoter

CMV

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) prME (JEV) sdm2

Plasmid

#52881

Purpose

expression of Japanese Encephalitis Virus VLPs in mammalian cells

Depositor

Yorgo Modis

Article

Nour et al PLoS Pathog. 2013;9(9):e1003585. doi: 10

Insert

prME (POLY Japanese encephalitis virus)

Expression

Mammalian

Mutation

sdm2

Promoter

CMV

Available Since

May 14, 2014

Availability

Academic Institutions and Nonprofits only
pH35C-gRNA-Cas9

Plasmid

#246794

Purpose

Express Cas9 protein and gRNA in plant cells (protoplast or callus) for Cas9-mediated editing

Depositor

Caixia Gao

Article

Li et al Nat Biotechnol. 2026 Jun 5. doi: 10.1038

Insert

gRNA, Cas9, HygR

Use

CRISPR

Available Since

March 17, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mScarlet-optoJNKi-WPRE

Plasmid

#159968

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mScarlet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-Ypet-optoJNKi-WPRE

Plasmid

#159967

Purpose

optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

Ypet-optoJNKi

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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TC1614

Plasmid

#164873

Purpose

CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A inserted into RfxCas13d (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 4NQ

Plasmid

#121487

Purpose

Expression of human PD-L1 4NQ with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Inserts

PD-L1 4NQ
hPD-L1 shRNA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

N35Q, N192Q, N200Q and N219Q

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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AAEL003877-Cas9

Plasmid

#100581

Purpose

The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL003877-hspcas9-GFP
Opie-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL003877 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pROSA26-Donor

Plasmid

#60952

Purpose

Porcine ROSA26 locus targeting

Depositor

Liangxue Lai

Article

Li et al Cell Res. 2014 Apr;24(4):501-4. doi: 10.

Insert

Porcine ROSA26 homologous ARM

Use

Gene targeting

Tags

Neo EGFP

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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NHA-Ago2

Plasmid

#36922

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

Ago2 (AGO2 Human)

Tags

NHA

Expression

Mammalian

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2Q2

Plasmid

#15801

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2Q2 (UBE2Q2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pCAT105

Plasmid

#180510

Purpose

Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
DpbCasX with R3 loop

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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