We narrowed to 8,695 results for: mcs

1. pET-28a+GFP-CAMSAP1 CKK

   Plasmid

   #59031

   Purpose

   expresses CKK domain of CAMSAP1 in E. coli

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP1 CKK domain (CAMSAP1 Human)
   

   Tags

   His, Strep, TEV, and sfGFP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pBJ1808 pRS416-COF1RFP

   Plasmid

   #37103

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Available Since

   June 19, 2012

   Availability

   Academic Institutions and Nonprofits only

3. 3535 pET15b Bid p22

   Plasmid

   #8784

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p22 (Bid Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. isoform PKC Alpha (JCE599)

   Plasmid

   #89779

   Purpose

   yeast expression of mouse PKC Alpha with yeast PKC1 promoter and ADH1 terminator

   Depositor

   Juan Carlos Igual

   Article

   Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

   Insert

   PKC Alpha (Prkca Mouse)
   

   Expression

   Mammalian

   Promoter

   yeast PKC1 promoter (-600 to -1)

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP Snail 2SA

   Plasmid

   #16226

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   Snail (SNAI1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   2SA: S97A, S101A

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLpC-L-IFP-F[1] (mammalian)

   Plasmid

   #52901

   Purpose

   Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[1]
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Unknown

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLpC-L-IFP-F[2] (mammalian)

   Plasmid

   #52902

   Purpose

   Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   L-IFP-F[2]
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   Unknown

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

8. AIRAP 8: pCEFL.PSMB2.GST

   Plasmid

   #21798

   Depositor

   David Ron

   Article

   Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

   Insert

   PSMB2 (Psmb2 Mouse)
   

   Tags

   GST

   Expression

   Mammalian

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-globin_del5UTR_PTC39-xrRNA-4H

   Plasmid

   #108367

   Purpose

   Expresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
   

   Expression

   Mammalian

   Mutation

   PTC39

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H

    Plasmid

    #108376

    Purpose

    FRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    PTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT-ChemoB-NAD

    Plasmid

    #193824

    Purpose

    CMV overexpression of ChemoB-NAD in mammalian cell lines

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoB-NAD
    

    Expression

    Mammalian

    Mutation

    EBFP2:N39Y, A206K, T225R; HT7: L271E

    Promoter

    CMV

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-TGF beta type I receptor (mL45)

    Plasmid

    #34701

    Depositor

    Ying Zhang

    Article

    Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N265A, D267A, N268A

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

13. Flag-TRIM24 shRescue

    Plasmid

    #28139

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    A489V; aa 308 and 309 mutated for shRNA rescue

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

    Plasmid

    #182345

    Purpose

    GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRibo-Tt

    Plasmid

    #154124

    Purpose

    Expresses wt Ribo-T rRNA and a set of tRNAs in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Inserts

    engineered bacterial rRNAs operon under control of pL promotor
    bacterial tRNAs (Asp, Trp, Ile, Ala, Glu) operon under control of Ptac promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptac and pL

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AIRAP 11: PSMD14.pEYFP.V1

    Plasmid

    #21801

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Sept. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP Snail 4SA

    Plasmid

    #16227

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    4SA: S108A, S112A, S116A, S120A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pJEC626

    Plasmid

    #174367

    Purpose

    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23106

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP Snail 2A4E

    Plasmid

    #16231

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    2A4E: S97A, S101A, S108E, S112E, S116E, S120E

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

20. Flag Snail 4SE

    Plasmid

    #16223

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    4SE: S108E, S112E, S116E, S120E

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only