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pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3-mRFP

Plasmid

#184902

Purpose

Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7, monomeric EGFP, and monomeric RFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ9824: CMV-SV40_NLS-CasMINI-V3.1--3xFlag- c-Myc_NLS-polyA

Plasmid

#176271

Purpose

Expression of CasMINI-V3.1 in mammalian cells.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI-V3.1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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miRFP670nano-pcDNA

Plasmid

#127443

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGH125_dCas9-Blast

Plasmid

#85417

Purpose

lentiviral expression vector for dCas9 with Blasticidin selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

dCas9 and Blasticidin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP

Plasmid

#172221

Purpose

Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19

Plasmid

#78597

Purpose

Express flag-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 (USP19 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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B-gTEMP/pcDNA3

Plasmid

#188447

Purpose

B-gTEMP for mammalian expression

Depositor

Takeharu Nagai

Article

Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

Insert

B-gTEMP

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

Plasmid

#203909

Purpose

Encodes wild type Gal4-VP64 transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4-VP64-IRES-mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-NanoLuc

Plasmid

#188313

Purpose

Expression of NanoLuc via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

NanoLuc

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RPS6 (pDD1902)

Plasmid

#52913

Purpose

contains RPS6 with HA-tag, which can be expressed in mammalian cells. Insert sites are at HindIII and BamHI.

Depositor

Dirk Dittmer

Article

Chen et al J Virol. 2011 Sep;85(18):9495-505. doi:

Insert

HAS6 (RPS6 Human)

Tags

HA

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-TRPT1

Plasmid

#184244

Purpose

For TRPT1 expression in mammalian cells

Depositor

Karla Feijs

Article

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm7.1

Plasmid

#210287

Purpose

Empty backbone with zeocin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAVU6+27-F30-2xdBroccoli

Plasmid

#66842

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Article

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Mammalian

Promoter

U6

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pAB1620 hU6-BpiI-Cas13bt3-DR

Plasmid

#176315

Purpose

hU6-BpiI-Cas13bt3-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt3 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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