We narrowed to 14,079 results for: UNG

1. pRS315-48nt-telo-I-SceI

   Plasmid

   #67623

   Purpose

   telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   48 bp telomere repeats
   hph
   

   Use

   Yeast cen/ars plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

   Plasmid

   #140204

   Purpose

   Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Step 1 of cloning c…

   Tags

   Expression

   Mutation

   Promoter

   Aspergillus fumigatus U3 (RNAPIII).

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

   Plasmid

   #101202

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3 variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   M1–N497, GGS, V713–D1368

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMEH60 Lamp2b MS2 HA

   Plasmid

   #168209

   Purpose

   Mammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   HA and MS2 dimer

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDSM-de-Pfba1-PYC-Trpl15a

   Plasmid

   #127738

   Purpose

   Yeast pathway position 5. PYC transcription unit with the FBA1 promoter and RPL15A terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   pyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pfba1

   Available since

   Jan. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315-0nt-telo-I-SceI

   Plasmid

   #67624

   Purpose

   telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   telomere cassette and I-SceI cut site
   hph
   

   Use

   Yeast cen/ars plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. myc-UVRAG S498D

   Plasmid

   #86739

   Purpose

   N-terminal myc-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human UVRAG (UVRAG Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S498D

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHL 1917

   Plasmid

   #53024

   Purpose

   AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

   Depositor

   Han Lim

   Article

   Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

   Inserts

   mCherry
   fimB (fimB E. coli)
   

   Use

   Synthetic Biology

   Tags

   Asp terminator, RBS(st7), and T1 terminator

   Expression

   Bacterial

   Mutation

   Promoter

   pCon and pLlacO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pDSM-ab-Psptef1-NAT-Ttip1

   Plasmid

   #127747

   Purpose

   Yeast pathway position 2. NatMX transcription unit with the S. paradoxus TEF1 promoter and TIP1 terminator. Nourseothricin resistance.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   nourseothricin N-acetyltransferase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Psptef1

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-UVRAG S498D

    Plasmid

    #86745

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S498D

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-UVRAG S498D

    Plasmid

    #86742

    Purpose

    N-terminal EGFP-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S498D

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TAL3071

    Plasmid

    #41245

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ets1a-Right (ets1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. TAL3070

    Plasmid

    #41244

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ets1a-Left (ets1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. CMYA5T2/pDEST15

    Plasmid

    #98109

    Purpose

    Express CMYA5 (aa 4039 – 4069) Leu allele (4063) with a N-terminal GST tag in bacteria

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    CMYA5 (CMYA5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    rs10043986 (NP_705838.3:p.Pro4063Leu)

    Promoter

    Available since

    Aug. 21, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. TAL3403

    Plasmid

    #47122

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-apoeb-right (apoeb Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

16. TAL3402

    Plasmid

    #47121

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-apoeb-left (apoeb Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.2 mir-1-1 reporter hsa-mir17~20

    Plasmid

    #46671

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    hsa-mir17~20 (MIR17 Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

    Plasmid

    #101213

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K855A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TAL3040

    Plasmid

    #41214

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-cebpa-Left (cebpa Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

20. TAL3041

    Plasmid

    #41215

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-cebpa-Right (cebpa Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only