We narrowed to 10,393 results for: epo

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EGFP-uTEV1-NES

Plasmid

#171006

Purpose

HiLITR protease with C-terminal NNES (Cytoplasmic)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-NES

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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PB_iETV2_P2A_GFP_Puro

Plasmid

#168805

Purpose

PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ETV2 (ETV2 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pH3mCHSH2

Plasmid

#101053

Purpose

This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

Depositor

Jennifer Lodge

Article

Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

Insert

Cryptococcus mCherry expression plasmid

Expression

Yeast

Promoter

Cryptococcus Histone3 promoter

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp38-C1

Plasmid

#134548

Purpose

Expresses YFP-Rpp38 fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Rpp38 (RPP38 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3_mScarlet-LAMP1

Plasmid

#185138

Purpose

Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

Depositor

Andrew York

Article

Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

Insert

Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)

Tags

mScarlet

Expression

Mammalian

Mutation

deleted amino acids 1-21 of LAMP1

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pPBbsr-JNK KTR-mCherry

Plasmid

#115493

Purpose

Expression vector for the JNK activity reporter JNK KTR-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

JNK KTR-mCherry

Tags

mCherry

Expression

Mammalian

Mutation

mCherry G229L, mCherry Y298 deleted

Promoter

CAG

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_LaG17-mCherry-HOTag3

Plasmid

#190024

Purpose

Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

Depositor

Lukasz Bugaj

Article

Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

Insert

LaG17 CluMPS

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-E SCT-T2A-GFP

Plasmid

#205461

Purpose

lentiviral plasmid for expression of HLA-E SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-E SCT (HLA-E Human)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT sfGPF 150TAG

Plasmid

#154766

Purpose

plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in GFP reporter, U25C in PylT

Promoter

EF1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-MLPmin-luc2

Plasmid

#194383

Purpose

Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

10xUAS-luc2

Expression

Mammalian

Promoter

MLP minimal

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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Oct4-IRES-eGFP-PGK-Neo

Plasmid

#48681

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Oct4 (Pou5f1 Mouse)

Use

CRISPR

Tags

GFP

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAG671 mito-pFAST

Plasmid

#172867

Purpose

Expresses mitochondrial targeting domain-pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

mito-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p2,0_3M5M_eGFP

Plasmid

#135474

Purpose

Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

Marburg virus minigenome, eGFP reporter

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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PB-Lmo2

Plasmid

#60666

Purpose

PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Lmo2 (Lmo2 Mouse)

Use

Piggybac

Expression

Mammalian

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215327

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

MUC5AC Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

MUC5AC

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro-CAG-GCaMP6s

Plasmid

#120896

Purpose

Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

Depositor

Xiaojun Lian

Article

Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

Insert

GCaMP6s

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215328

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

FOXJ1 Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

FOXJ1

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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