We narrowed to 32,111 results for: Eng

1. pAAV-hSyn-GACh2.0

   Plasmid

   #121921

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GACh2.0

   Depositor

   Yulong Li

   Article

   Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

   Insert

   GPCR activation based ACh sensor GACh2.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ262-ABE8e

   Plasmid

   #161523

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   ABE8e-zSpCas9(D10A)
   

   Use

   CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

   Plasmid

   #178518

   Purpose

   For making Rabies virus SAD B19 strain L cell line with hyPBase

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   B19L-IRES-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265E6

   Plasmid

   #213469

   Purpose

   Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

   Depositor

   Yiping Qi

   Article

   Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

   Insert

   TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
   

   Use

   CRISPR; Gateway compatible tada-cda-32aa linker-z…

   Tags

   No

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ266E

   Plasmid

   #161521

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 PmCDA1 mediated C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   zSpRYCas9(D10A)-PmCDA1-UGI
   

   Use

   CRISPR; Gateway compatible zsprycas9(d10a)-pmcda1…

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ262B-ABE8e

   Plasmid

   #161524

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

   Insert

   TadA8e-zSpRYCas9(D10A)
   

   Use

   CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSUPER-retro-puro-shOrai1

   Plasmid

   #89933

   Purpose

   Knockdown human Orai1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   Orai1 shRNA (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PITX3-eGFP-PGK-Puro

   Plasmid

   #31942

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Insert

   PITX3 (PITX3 Human)
   

   Use

   Targeting donor

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag2-Sox9 T236A

   Plasmid

   #111455

   Purpose

   Transient expression of Flag-tagged human Sox9 with T236A mutation

   Depositor

   Sharon Pine

   Article

   Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

   Insert

   Sox9 (SOX9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T236A mutation

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDisplay-GACh1.0

    Plasmid

    #106072

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUPER-retro-puro-shSTIM1

    Plasmid

    #89816

    Purpose

    Knockdown human STIM1 expression

    Depositor

    Shengyu Yang

    Article

    Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

    Insert

    STIM1 shRNA (STIM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

    Plasmid

    #99229

    Purpose

    Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos2
    

    Use

    Tags

    Lifeact

    Expression

    Mammalian

    Mutation

    A69T for primed conversion applications

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUPER-retro-puro-Smad4

    Plasmid

    #89829

    Purpose

    Knockdown human Smad4 expression in human mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

    Insert

    Smad4 shRNA (SMAD4 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Tag2-Sox9 T240A

    Plasmid

    #111457

    Purpose

    Transient expression of Flag-tagged human Sox9 with T240A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T240A

    Promoter

    CMV

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSET-pr-mKikGR1 (mKikGR1-V69T)

    Plasmid

    #99227

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mKikGR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    monomeric variant with mutation V69T (Eos notatio…

    Promoter

    T7

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG-mGSDMD1-251

    Plasmid

    #111565

    Purpose

    Express ELANE-cleaved mouse GSDMD in mammalian cells

    Depositor

    Hongbo Luo

    Article

    Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

17. pCMV-Tag2-Sox9 T236A/T240A

    Plasmid

    #111458

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A/T240A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A and T240A

    Promoter

    CMV

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. B4GALT1-Dox

    Plasmid

    #124633

    Purpose

    Doxycycline-inducible B4GALT1 expression in mammalian cells

    Depositor

    Ron Weiss

    Article

    Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

    Inserts

    Beta-(1,4)-Galactosyltransferase 1
    Tetracycline repressor A3 mutant
    TagFP635
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-tight and hEF1a

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBRPyCAG-fmKlf4-DsRed-Ip

    Plasmid

    #26274

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge