We narrowed to 4,451 results for: Gca

1. pZac2.1 U6-SaAi9L-U6-SaAi9R

   Plasmid

   #78604

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   gRNAs for SaCas9 targeting Ai9 locus
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   Behnam Nabet, James Bradner

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-puro-irf3-shrna1

   Plasmid

   #127648

   Purpose

   Knock-down of human IRF3

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 shRNA (IRF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

   Plasmid

   #221233

   Purpose

   Express sgRNA targeting HEK3 loci with nCas9(D10A)

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(D10A)-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-R-GECO1

   Plasmid

   #32444

   Purpose

   Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   R-GECO1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Substitutions relative to the mApple-derived anal…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-LAMP1

   Plasmid

   #207787

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. RNASEL gRNA (BRDN0001149212)

   Plasmid

   #77051

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RNASEL

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RNASEL (RNASEL Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

   Plasmid

   #214881

   Purpose

   Lentiviral vector encoding RfxCas13d targeting B2M guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crB2M-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458_KMT2B_2

   Plasmid

   #101075

   Purpose

   Encodes gRNA for 3' target of human KMT2B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KMT2B (KMT2B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-NLS-R-GECO

    Plasmid

    #32462

    Purpose

    Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    R-GECO1.0
    

    Use

    Tags

    NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Substitutions relative to the mApple-derived anal…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. LENTICRISPR-UPP1_sgRNA2

    Plasmid

    #201635

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    UPP1 (UPP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. shRNA_circHUWE1_1

    Plasmid

    #215236

    Purpose

    Supression of shcircHUWE1(19,20)_1 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    circHUWE1 shRNA 1 (HUWE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. SPHK2 gRNA (BRDN0001148465)

    Plasmid

    #77093

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK2 (SPHK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-itis pBE-nt

    Plasmid

    #195343

    Purpose

    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Inserts

    ecTadA(8e)-SpCas9-NG
    gRNA gtgcacgacgccgtatgcga
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

    Promoter

    Lac and ProC

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9nucl-T2A-mCherry - BAKgRNA1- BAKgRNA2

    Plasmid

    #167295

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAK gene and a CMV driven nuclease Cas9 followed by self-cleaving mCherry

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAK (BAK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 puro_shDDX58_230212

    Plasmid

    #167293

    Purpose

    Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro resistance cassette.

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    shRNA hsDDX58 (RIGI Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORANGE Tubb3-GFP KI

    Plasmid

    #131497

    Purpose

    Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCIP-hCD69-TdTomato

    Plasmid

    #154095

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Inserts

    [5HA]-P2A-tdTomato-[3HA]
    hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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