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We narrowed to 63,044 results for: cloning

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Showing: 2941 - 2960 of 63044 results

  2. pSBbi-BH

    Plasmid
    #60515
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    60515-plasmid-map-sequence-id-97531

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  11. R777-E140 Hs.NF1-nostop

    Plasmid
    #70424
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    70424-plasmid-map-sequence-id-125873

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  12. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

    Plasmid
    #161926
    Purpose
    Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315
    Insert
    rTetR(SE-G72P)
    Use
    Lentiviral and Synthetic Biology
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    pEF
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    161926-plasmid-map-sequence-id-324346

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  14. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49235
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49235-plasmid-map-sequence-id-376544

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  18. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Publication
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. R777-E173 Hs.PIK3R2

    Plasmid
    #70457
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70457-plasmid-map-sequence-id-125906

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Showing: 2941 - 2960 of 63044 results