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We narrowed to 60,787 results for: cloning

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Showing: 2941 - 2960 of 60787 results

  6. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  8. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Expression
    Mammalian
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  10. p3E-MCS

    Plasmid
    #75174
    Purpose
    Multiple cloning site for insertion of gene of interest for C-terminal fusions.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    Multiple Cloning Site
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75174-plasmid-map-sequence-id-142182

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  12. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  13. Ccas9LacZ

    Plasmid
    #113083
    Purpose
    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Worm
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113083-plasmid-map-sequence-id-218959

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  15. pG108-K - AhpC-Glow

    Plasmid
    #185135
    Purpose
    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoter
    Depositor
    Janina Lewis
    Article
    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1
    Inserts
    Glow gene
    Bacteroides thetaiotaomicron ahpC promoter
    Use
    Pg106
    Expression
    Bacterial
    Available Since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    185135-plasmid-map-sequence-id-378889

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  16. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Promoter
    pPH (Polyhedrin promoter)
    Available Since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

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  17. pAC-ZEAX

    Plasmid
    #53274
    Purpose
    Contains crtE, crtB, crtI, crtY and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52
    Insert
    crtE, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Mutation
    Deleted idi (ORF6) and crtX genes
    Promoter
    endogenous promoters
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53274-plasmid-map-sequence-id-83289

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Showing: 2941 - 2960 of 60787 results