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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,355 results for: actin

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Showing: 2961 - 2980 of 3355 results

  2. psBIG1a ActBT2ATetP2AP - mAGH

    Plasmid
    #229973
    Purpose
    Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-Hygro
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    ActBT2ATetP2AP - mAGH
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229973-plasmid-map-sequence-id-461257

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  11. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  12. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

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  13. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

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  15. pAct-GST-H3

    Plasmid
    #182842
    Purpose
    A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    GST-histone H3 fusion
    Tags
    GST
    Expression
    Insect
    Promoter
    Actin5C
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182842-plasmid-map-sequence-id-360059

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  18. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Available Since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98306-plasmid-map-sequence-id-181809

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Showing: 2961 - 2980 of 3355 results