We narrowed to 26,563 results for: Cat

1. SpyTag-β-Lactamase-SpyCatcher

   Plasmid

   #52656

   Purpose

   Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

   Depositor

   Mark Howarth

   Article

   Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

   Insert

   TEM-1 β-Lactamase
   

   Use

   Tags

   SpyCatcher and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CEL-IFP2-SOScat

   Plasmid

   #208746

   Purpose

   catalytic domain of SOS2 fused with near ir fluorescent protein and CEL tag

   Depositor

   Xiaokun Shu

   Article

   Chung et al ACS Cent Sci. 2023 Jul 7;9(7):1466-1479.

   Insert

   CEL-IFP2-SOScat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p15A-OptoCre-cat-P-R

   Plasmid

   #189853

   Purpose

   Cre-inducible activation of cat for chloramphenicol resistance.

   Depositor

   Mary Dunlop

   Article

   Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

   Insert

   P-loxP-TT-loxP-R-cat
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P (T7 phage gene 10)

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCXIP-cathepsinD-YMH

   Plasmid

   #182644

   Purpose

   Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   cathepsinD
   

   Use

   Retroviral

   Tags

   His6-tag, TS site from proCCK, and myc-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. His6-SpyCatcher-mi3

   Plasmid

   #113043

   Purpose

   Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   His6-SpyCatcher-mi3
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1-CAT

   Plasmid

   #173943

   Purpose

   Bacterial expression of isolated PARP1 CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST14-DoubleCatcher-H-Lock

   Plasmid

   #216285

   Purpose

   Expresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher H-Lock
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-LgBiT-SpyCatcher

   Plasmid

   #223634

   Purpose

   Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Use

   Tags

   His6, LgBiT

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28_6xHis-PARP1-CATdeltaHD

    Plasmid

    #174811

    Purpose

    Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CATdeltaHD (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Residues 678-787 replaced by eight-residue linker…

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET30a-His6-LOXCATmut

    Plasmid

    #140085

    Purpose

    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

    Depositor

    Vamsi Mootha

    Article

    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Insert

    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Mutation in H265A and R268A in LOX (used original…

    Promoter

    T7

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-α-cateninA+ΔβH

    Plasmid

    #229704

    Purpose

    Transient expression of GFP-alpha-cateninA+deltabetaH in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 670-673, RAIM--> GSGS

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKC alpha CAT

    Plasmid

    #21234

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta 1-325

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCGT-Sos Cat

    Plasmid

    #32923

    Depositor

    Dafna Bar-Sagi

    Article

    Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

    Insert

    SOS1 (SOS1 Human)
    

    Use

    Tags

    T7 epitope

    Expression

    Mammalian

    Mutation

    Contains aa 564-1049 (catalytic domain)

    Promoter

    CMV

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pDEST14-Cys-SpyCatcher002

    Plasmid

    #102829

    Purpose

    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Cys-SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Cysteine residue in N-terminal region

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. modified pcAT7-Glo1

    Plasmid

    #160996

    Purpose

    Splicing reporter backbone

    Depositor

    Brenton Graveley

    Article

    Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

    Insert

    hemoglobin subunit beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Repeated intron 1 with 40 nucelotide deletion

    Promoter

    CAG

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFN18A-HaloTag-SpyCatcher

    Plasmid

    #206041

    Purpose

    HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    HaloTag and SpyCatcher-8His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. hE-cadherin/Δβ-catenin-pcDNA3

    Plasmid

    #45772

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Δβ-catenin (CDH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ends at amino acid 844. Last 35 amino acids dele…

    Promoter

    CMV

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWP78-PVCpnf_pvc13-truncated

    Plasmid

    #198286

    Purpose

    Contains a tail fiber with a truncated binding domain (lacking aa403-476 in Pvc13); produces nontargeting PVCs

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-truncated
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLViN-iRFP670-α-cateninWT

    Plasmid

    #229702

    Purpose

    Lentiviral expression of iRFP670-alpha-cateninWT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    CTNNA1 (alpha-catenin) Human (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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