We narrowed to 14,093 results for: GAN

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Strain CSH100

Bacterial Strain

#21875

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Bacterial Resistance

None

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (Zeocin)

Species

Escherichia coli

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available Since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pFastbac-Flag-BRCA1

Plasmid

#223228

Purpose

Insect expression construct for Flag-tagged BRCA1 protein production and purification

Depositor

Weixing Zhao

Article

Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

Insert

Breast Cancer 1 (BRCA1 Human)

Tags

Flag

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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BW-Para

Bacterial Strain

#172602

Purpose

The BW-Para E. coli strain is used to screen the functions of chimeric AraC/XylS transcription activators using beta-galactosidase assays.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Bacterial Resistance

None

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only
pTL

Plasmid

#203176

Purpose

Centromeric yeast expression vector, leucine selection

Depositor

William Sessa

Article

Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTL-DHDDS

Plasmid

#203177

Purpose

Centromeric yeast expression vector for hDHDDS

Depositor

William Sessa

Article

Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

Insert

DHDDS (DHDDS Human)

Expression

Yeast

Promoter

RER2

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Strain: C321.ΔA

Bacterial Strain

#68306

Purpose

Genomically recoded strain of E. coli (no genomic TAG)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Bacterial Resistance

Bleocin (Zeocin)

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
pBS cPitx2 (CT#73)

Plasmid

#13927

Depositor

Cliff Tabin

Article

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
RCAS (A) Flu cPitx2 (CT#704)

Plasmid

#13928

Depositor

Cliff Tabin

Article

Logan et al Cell. 1998 Aug 7. 94(3):307-17.

Insert

pitx2 (PITX2 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
KG#707

Plasmid

#110911

Purpose

Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

Insert

sup-1 minigene

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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SP6-hCas9-Ce-mRNA

Plasmid

#47911

Purpose

In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

Depositor

Paul Sternberg

Article

Chiu et al Genetics. 2013 Aug 26.

Insert

hCas9 with C. elegans 5' and 3' UTRs

Use

CRISPR

Promoter

SP6

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pET-UNC60B

Plasmid

#48740

Purpose

Bacterial expression of UNC-60B (a C. elegans ADF/cofilin)

Depositor

Shoichiro Ono

Article

Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Insert

C. elegans UNC-60B cDNA (unc-60 Nematode)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET-UNC60A

Plasmid

#48739

Purpose

Bacterial expression of UNC-60A (a C. elegans ADF/cofilin)

Depositor

Shoichiro Ono

Article

Ono et al J Biol Chem. 1998 Feb 6;273(6):3778-83.

Insert

C. elegans UNC-60A cDNA (unc-60 Nematode)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-HA-DRE-1-pcDNA3.1-

Plasmid

#52513

Purpose

expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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