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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 281 - 300 of 3270 results

  2. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72248-plasmid-map-sequence-id-131868

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  3. pPEPX-P3-sgRNAluc

    Plasmid
    #85590
    Purpose
    Integrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template for
    Depositor
    Jan-Willem Veening
    Publication
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    sgRNA targeting firefly luciferase encoding gene
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85590-plasmid-map-sequence-id-169513

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  12. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid
    #101209
    Purpose
    Bacterial expression plasmid for SpCas9-HF1 variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101209-plasmid-map-sequence-id-198127

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  13. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58694-plasmid-map-sequence-id-90691

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  16. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58693-plasmid-map-sequence-id-492918

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  17. px459 VQR

    Plasmid
    #101715
    Purpose
    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q and T1337R)
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101715-plasmid-map-sequence-id-196971

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  18. PX403 Streptococcus thermophilus LMD-9 CRISPR 3 Cas9

    Plasmid
    #68337
    Purpose
    Streptococcus thermophilus LMD-9 CRISPR 3 Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    St3Cas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68337-plasmid-map-sequence-id-125198

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  19. p201H Cas9

    Plasmid
    #59176
    Purpose
    Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle
    Depositor
    Wayne Parrott
    Publication
    Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    hph
    Use
    CRISPR
    Promoter
    2x35S and StUbi-3P
    Available since
    Aug. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59176-plasmid-map-sequence-id-120424

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Showing: 281 - 300 of 3270 results