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pLentiPGK Puro DEST JNKKTRClover

Plasmid

#59151

Purpose

Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP3

Plasmid

#42310

Depositor

Thierry Galli

Article

Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

Insert

VAMP 3 (VAMP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2013

Availability

Academic Institutions and Nonprofits only

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DsRed-rab9 DN

Plasmid

#12676

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pBabe puro Snail

Plasmid

#23347

Depositor

Bob Weinberg

Article

Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

Insert

Snail (SNAI1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only

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EGFP-E-Syt1

Plasmid

#66830

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLJM1-FIRE-pHLy

Plasmid

#170775

Purpose

Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

Depositor

Aimee Kao

Article

Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

Insert

LAMP1 (LAMP1 Human)

Use

Lentiviral

Tags

mCherry and mTFP1

Expression

Mammalian

Mutation

84 nucleotide Signal Sequence switched to the beg…

Promoter

CMV

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA

Plasmid

#154867

Purpose

Flp-dependent hM4D(Gi) DREADD-mCherry

Depositor

Ulrik Gether

Article

Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03

Has Service

AAV Retrograde and AAV8

Insert

hM4D(Gi)-mCherry

Use

AAV; Flp-frt

Tags

mCherry

Expression

Mammalian

Promoter

Human Synapsin-1

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Ec1

Plasmid

#138474

Purpose

Enables high-efficiency recombineering in E. coli

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

CspRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-HNRNPU

Plasmid

#38068

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shNS

Plasmid

#127696

Purpose

Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shNS

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

Plasmid

#138147

Purpose

Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas13-M3nls

Plasmid

#155366

Purpose

Targeted m6A RNA methylation in mammalian cells

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Use

CRISPR

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Article

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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MaLionR

Plasmid

#113908

Purpose

Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL-Neo-Myr-Flag-DEST

Plasmid

#15300

Purpose

a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

Depositor

Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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eeBxb1

Plasmid

#222339

Purpose

Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP

Plasmid

#57823

Purpose

3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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mApple-pBAD

Plasmid

#54536

Purpose

Localization: Protein Expression Vector , Excitation: 568, Emission: 592

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

6xHis and mApple

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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