We narrowed to 24,337 results for: Spr

1. pDL28_Cas9-His Δcys E532C/E1207C

   Plasmid

   #206289

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDL25_Cas9-His Δcys E1207C

   Plasmid

   #206286

   Purpose

   Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1207C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDL23_Cas9-His Δcys E945C

   Plasmid

   #206284

   Purpose

   Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDL24_Cas9-His Δcys E1026C

   Plasmid

   #206285

   Purpose

   Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, C574S, E1026C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDL27_Cas9-His Δcys E532C/E945C

   Plasmid

   #206288

   Purpose

   Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S, E945C

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDL26_Cas9-His Δcys M1C/E532C

   Plasmid

   #206287

   Purpose

   Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   M1C, C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDL22_Cas9-His Δcys E532C

   Plasmid

   #206283

   Purpose

   Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

   Depositor

   Amit Choudhary

   Article

   Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   6x-His and SV40 NLS

   Expression

   Bacterial

   Mutation

   C80S, E532C, C574S

   Promoter

   T7

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lenti-Cas9-sgHPRT1

   Plasmid

   #196713

   Purpose

   CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

   Depositor

   Bernhard Schmierer

   Article

   Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

   Insert

   Cas9-T2A-BSD-U6-sgHPRT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a/hU6

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRnc-gGFP129

   Plasmid

   #189537

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-129-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DpbdCas12e KRAB

    Plasmid

    #188505

    Purpose

    Expresses FLAG tagged DpbdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D672A/E769A/D935A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. NAX_CAGGS_CAS9_m4_mcherry

    Plasmid

    #167870

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

12. pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179010

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179009

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)

    Plasmid

    #179080

    Purpose

    Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

    Plasmid

    #179026

    Purpose

    Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

    Plasmid

    #179025

    Purpose

    Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

    Plasmid

    #179024

    Purpose

    Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179023

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179022

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179021

    Purpose

    Parent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge