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We narrowed to 63,812 results for: cloning

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Showing: 2981 - 3000 of 63812 results

  2. pMA-SpCas9-g1

    Plasmid
    #80784
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80784-plasmid-map-sequence-id-162402

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  5. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Publication
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. R777-E173 Hs.PIK3R2

    Plasmid
    #70457
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70457-plasmid-map-sequence-id-125906

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  12. R777-E033 Hs.DUSP1

    Plasmid
    #70317
    Purpose
    Gateway ORF clone of human DUSP1 [NM_004417.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP1 (DUSP1 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70317-plasmid-map-sequence-id-125766

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  13. pMA-SpCas9-g2

    Plasmid
    #80785
    Purpose
    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array
    Depositor
    Yonglun Luo
    Publication
    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPR gRNA expression cassette (for SpCas9)
    Use
    CRISPR
    Tags
    na
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    80785-plasmid-map-sequence-id-162403

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  16. p5E-Runx1+23

    Plasmid
    #69602
    Purpose
    Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning
    Depositor
    Owen Tamplin, Leonard Zon
    Publication
    Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:
    Inserts
    Runx1 +23 intronic enhancer (Runx1 Mouse)
    Mouse beta-globin minimal promoter
    Use
    5' gateway entry vector
    Promoter
    C57/BL6 mouse Runx1 +23 intronic enhancer and C57…
    Available since
    Sept. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69602-plasmid-map-sequence-id-190603

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  17. MSCV-mMDM2-IRES-GFP

    Plasmid
    #66954
    Purpose
    retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site
    Depositor
    Martine Roussel
    Insert
    Mdm2 (Mdm2 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Promoter
    MSCV
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66954-plasmid-map-sequence-id-173086

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  18. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Depositor
    Nicolas Charlet-Berguerand
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NOVA1 (NOVA1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61275-plasmid-map-sequence-id-99586

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Showing: 2981 - 3000 of 63812 results