We narrowed to 8,876 results for: sgrna
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Plasmid#61253PurposeTemplate for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusionDepositorInsertU6 promoter
UseCRISPR; Cloning templateAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
LBJJ383
Plasmid#117517PurposePCR template for sgRNA classic with polyT as terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT58_X2site
Plasmid#177705PurposeExpresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL5-pegRNA-attR3
Plasmid#213054PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3DepositorInsertsgRNA
ExpressionBacterialAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
p458 VQR
Plasmid#101727PurposeExpresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifsDepositorInsertSpCas9 VQR
ExpressionMammalianMutationVQRAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAS9-BFP empty
Plasmid#80976PurposeCas9, TagBFP, and sgRNA expressionDepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgPal7 (p2Tol-U6-sgPal7-HygR)
Plasmid#71484PurposeExpresses palindromic sgRNA for use in self-cloning CRISPRDepositorInsertsgPal7
PromoterU6Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB70
Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRExpressionWormAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpyB
Plasmid#220186PurposesgRNA expression vector - pBG35 promoterDepositorInsertpBG35 promoter
UseCRISPRExpressionBacterialPromoterpBG35Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-U6-Xba1
Plasmid#203300PurposeFor expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
px330 SUZ12 - pDY083
Plasmid#171109PurposeExpresses Cas9 and an sgRNA targeting SUZ12DepositorInsertCas9
ExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 EZH2 - pDY052
Plasmid#171107PurposeExpresses Cas9 and an sgRNA targeting EZH2DepositorInsertCas9
ExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDCC5
Plasmid#59984PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassetteDepositorInsertCas9-D10A
UseCRISPRTagsHAExpressionInsectMutationD10APromoterhsp70BbAvailable SinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTJV1ScKm-rpoB
Plasmid#166980PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistanceDepositorInsertNeoR/KanR
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC2103-reci
Plasmid#117172PurposeU6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLSDepositorInsertreci_arCas9 without NLS
UseLentiviralAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA4-RFP
Plasmid#62250Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA4Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMB60
Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorInsertsynthetic guide RNA
UseCRISPRExpressionWormAvailable SinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA2-RFP
Plasmid#62248Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA2Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJF131B
Plasmid#113326PurposepTet-W108 scRNA.b2, pTet-RR2-sgRNADepositorInsertW108 scRNA_MS2 and sgRNA_RR2
UseCRISPRExpressionBacterialAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
px459 EQR
Plasmid#101732PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifsDepositorInsertSpCas9 EQR
ExpressionMammalianMutationVQRAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
U6>XX
Plasmid#87542PurposeComponent 1 of 2 for U6>sgRNA cassette OSO-PCRDepositorTypeEmpty backboneAvailable SinceJan. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_IL50_HOlocus
Plasmid#194426PurposeExpresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiaeDepositorInsertCas9
ExpressionYeastMutationNAAvailable SinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgPal1 (p2Tol-U6-sgPal1-HygR)
Plasmid#71483PurposeExpresses palindromic sgRNA for use in self-cloning CRISPRDepositorInsertsgPal1
PromoterU6Available SinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA1-RFP
Plasmid#62247Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA1Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-7SK-Blast
Plasmid#183620PurposelentiCRISPRv2 derivative with modified 7SK promoter driving sgRNA and encoding Blasticidin resistance. Compatible with inserts designed for lentiCRISPRv2.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterModified 7SKAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN-PTet-YFP
Plasmid#62245Purposeused to characterize the promoter activities of PTet for dCas9 and sgRNA response functions.DepositorInsertYFP
ExpressionBacterialPromoterPtetAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
YN2_1_LT84_RPL13A
Plasmid#177712PurposeExpresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiaeDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAN-PA3-RFP
Plasmid#62249Purposereporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1DepositorInsertmRFP
ExpressionBacterialPromoterpA3Available SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBC2101-reci
Plasmid#117170PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLSDepositorInsertreci_wtCas9 with SV40 NLS
UseLentiviralAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-YFP
Plasmid#62246Purposeused to characterize the promoter activities of PBAD for dCas9 and sgRNA response functions.DepositorInsertYFP
ExpressionBacterialPromoterpBADAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 - sgAP2S1 #2
Plasmid#202757PurposeEncodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1DepositorInsertgRNA targeting AP2S1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 - sgAP2M1
Plasmid#202758PurposeEncodes Doxycycline inducible Cas9 and sgRNA targeting AP2M1DepositorInsertgRNA targeting AP2M1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 - sgAP2S1 #1
Plasmid#202756PurposeEncodes Doxycycline inducible Cas9 and sgRNA targeting AP2S1DepositorInsertgRNA targeting AP2S1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2ATetP2AP - mAGH
Plasmid#229973PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and mAGNLS-T2A-HygroDepositorInsertActBT2ATetP2AP - mAGH
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2ATetP2AP - H
Plasmid#229972PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and HygroDepositorInsertActBT2ATetP2AP - H
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
psBIG1a ActBT2AmcherryP2AP -TagBFPH
Plasmid#229971PurposeHarbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-HygroDepositorInsertActBT2AmcherryP2AP -TagBFPH
UseCRISPRExpressionMammalianAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA47_dCas9-GFP_NOT_i1
Plasmid#231416PurposeThis NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.DepositorInsertsdCas9
GFP
UseSynthetic BiologyAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpy
Plasmid#220184PurposesgRNA expression vector - promoterless backboneDepositorInsertpromoterless backbone
UseCRISPRExpressionBacterialPromoterpromoterlessAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA
Plasmid#220185PurposesgRNA expression vector - pEM7 promoterDepositorInsertpEM7 promoter
UseCRISPRExpressionBacterialPromoterpEM7Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only