We narrowed to 4,369 results for: 1-Oct

1. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-hPLXNB2_PGK_Neo

   Plasmid

   #176848

   Purpose

   lentiviral vector for constitutive PLXNB2 overexpression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hPLXNB2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   full length PLXNB2

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKP332 (Lenti-OSK1)

   Plasmid

   #21627

   Depositor

   Tim Townes

   Article

   Chang et al Stem Cells. 2009 May . 27(5):1042-9.

   Insert

   hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUW-VenusFlag-hCD44

   Plasmid

   #211824

   Purpose

   Express CD44 in mammalian cells

   Depositor

   Haian Fu

   Article

   Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

   Insert

   CD44 (CD44 Human)
   

   Use

   Lentiviral

   Tags

   N-Terminal Venus and Flag tags

   Expression

   Mammalian

   Mutation

   isoform corresponds to UniProt ID P16070-1 with a…

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

   Plasmid

   #176851

   Purpose

   lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   hYAP1-5SA (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   S61A, S109A, S127A, S164A, S381A (phosphorylation…

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDmelOR-mRho.V5.mER.hOr47a

   Plasmid

   #126478

   Purpose

   Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Inserts

   Or47a (Or47a Fly)
   Orco (Orco Fly)
   

   Use

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens and codon optim…

   Promoter

   CMV

   Available since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. t1-405(del37/GAG)

   Plasmid

   #166129

   Purpose

   Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   T1head1-405(del37/GAG) (TLN1 Human)
   

   Use

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   aa1-405, with 37 aa deletion in the F1-loop which…

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

   Plasmid

   #223959

   Purpose

   Dual fluorescent reporter for histone H2B and for APC/C activity

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-BOS-RIG-I-Myc

   Plasmid

   #215872

   Purpose

   RIG-I-Myc expression vector for overexpression

   Depositor

   Kumiko Ui-Tei

   Article

   Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

   Insert

   RIG-I (RIGI Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-hERG1a

    Plasmid

    #53052

    Purpose

    Expresses alpha subunit of hERG1a K channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. PH-Btk-GFP

    Plasmid

    #51463

    Purpose

    Is a biosensor for PIP3

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    BTK (aa 1–177) (BTK Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAL113

    Plasmid

    #22471

    Purpose

    Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PhyB NT (PHYB Mustard Weed, Mouse)
    

    Use

    Synthetic Biology

    Tags

    10aaLinker-mCFP-HrasCT

    Expression

    Mammalian

    Mutation

    Y276H

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET15b His-p97

    Plasmid

    #169020

    Purpose

    Expresses His-p97 in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Tags

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDsRed Monomer C1-YAP2

    Plasmid

    #19057

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Red-FPP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-GRK6

    Plasmid

    #32693

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK6 (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-FOXG1

    Plasmid

    #153124

    Purpose

    Expression of Flag tagged human FOXG1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXG1 (FOXG1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE DN Cullin5

    Plasmid

    #41916

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    CUL5 (CUL5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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