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lenti-HA-mBec1

Plasmid

#99505

Purpose

3rd generation lentiviral expression of wild-type Becn1

Depositor

Congcong He

Article

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-ABP-LOXCATmut

Plasmid

#139972

Purpose

A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

Depositor

Vamsi Mootha

Article

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Tags

His6

Expression

Bacterial

Mutation

Mutation in H265A and R268A in LOX (used original…

Promoter

T7

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBC2T-actin

Plasmid

#108968

Purpose

expresses BC2-tag-actin in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

beta-actin (ACTB Human)

Tags

BC2-tag

Expression

Mammalian

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-KD

Plasmid

#114045

Purpose

Expression of exogenous LAP tagged MPS1 Kinase-dead

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-KD (TTK Human)

Expression

Mammalian

Mutation

D664A kinase dead

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pR26-mBMP7

Plasmid

#127374

Purpose

Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

BMP7 (Bmp7 Mouse)

Use

CRISPR and Mouse Targeting

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Beclin-BATS

Plasmid

#30498

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Beclin, Barkor (BECN1, ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

Beclin 1 + 413-492 aa of Barkor

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pmKikGR Actin

Plasmid

#105315

Purpose

Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

Depositor

Kenneth Yamada

Article

Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

Insert

actin beta (ACTB Human)

Tags

KikGR

Expression

Mammalian

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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F2R-bio-His

Plasmid

#51863

Purpose

Expresses full-length Proteinase-activated receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

F2R (F2R Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Actin-7

Plasmid

#55085

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-ms-c-Myc-V394D

Plasmid

#50775

Purpose

retroviral expression of mouse c-Myc V394D mutant

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc V394D (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

V394D (does not bind to Miz-1)

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRCreb5gRNA

Plasmid

#195021

Purpose

Sequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domain

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Creb5 gRNA (CREB5 Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCOQ2

Plasmid

#186025

Purpose

knock out COQ2 in mammalian cells

Depositor

Boyi Gan

Article

Mao et al Nature. 2021 May;593(7860):586-590. doi:

Insert

Coenzyme Q2, Polyprenyltransferase (COQ2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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miniSOG-Actin-7

Plasmid

#57761

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TFORF1395

Plasmid

#143106

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF569 (ZNF569 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO GFP MPS1 K-R

Plasmid

#59819

Purpose

Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

Insert

Mps1 (TTK Human)

Tags

EmGFP

Expression

Mammalian

Mutation

KD, catalytically inactive, D664A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-c-Myc-dN2

Plasmid

#50774

Purpose

retroviral expression of human c-Myc dN2 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc -dN2 (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 42-439

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-c-Myc-dN1

Plasmid

#50773

Purpose

retroviral expression of human c-Myc dN1 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc -dN1 (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 14-439

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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