We narrowed to 34,883 results for: CaS

1. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCASP-HilA empty vector

   Plasmid

   #153333

   Purpose

   Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Type

   Empty backbone

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

   Plasmid

   #107305

   Purpose

   Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2x NLS and FKBP

   Expression

   Mammalian

   Mutation

   R1335K and fused to FKBP

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET_His6-MBP-TEV-Cas2

   Plasmid

   #186275

   Purpose

   Expresses Thiomicrospira Cas2 in E. coli

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicropira Cas2
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCasper-pNos-PCP-eGFP

   Plasmid

   #180274

   Purpose

   to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

   Depositor

   Hernan Garcia

   Article

   Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

   Insert

   PCP-eGFP
   

   Use

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCASP-SptP120-3m160-HilA

   Plasmid

   #153325

   Purpose

   Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-3m160 DARPin
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. (517) SB KRAB-SadCas9 U6-gStop

   Plasmid

   #163023

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMuLE ENTR SV40-hCas9 L3-L2

   Plasmid

   #62133

   Purpose

   MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning

   Depositor

   Ian Frew

   Article

   Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

   Insert

   human codon optimized Cas9
   

   Use

   CRISPR; Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-Flag-eSpCas9-H840A

   Plasmid

   #80454

   Purpose

   enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   ehSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9-Puro HLAG-2B-sgRNA

    Plasmid

    #182552

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2B-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. RCASBP(A)-3xFlag-mHoxd13+7Ala

    Plasmid

    #145289

    Purpose

    Avian Expression of murine SPDH (+7Ala) Hoxd13 with N-terminal 3xFlag tag

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    Hoxd13(+7Ala) (Hoxd13 Mouse)
    

    Use

    Viral avian expression

    Tags

    3xFlag

    Expression

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

    Plasmid

    #148855

    Purpose

    Mammalian Expression of HsAGO3-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO3-sgRNA (AGO3 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

    Plasmid

    #149590

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM116: CasRx VEGFA presgRNA

    Plasmid

    #166868

    Purpose

    U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. A3H HapII-Cas9n-UGI

    Plasmid

    #119141

    Purpose

    Base editor made from APOBEC3H Haplotype II

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype II-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. RCAS(A) dRhoA N19 (CT#847)

    Plasmid

    #14002

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

    Insert

    RhoA (Rho1 Fly)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    T19N

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA pDEST40 Cas9 GW STOP

    Plasmid

    #183196

    Purpose

    Destination vector

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid

    #121961

    Purpose

    Mammalian expression, Transcription regulation, deactivated AaCas12b

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    Catalytically deactivated AaCas12b
    

    Use

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    R911A

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX544 SpCas9(del1098-1368)

    Plasmid

    #58889

    Purpose

    Truncated form of SpCas9 without C' domain

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    del amino acids 1098-1368

    Promoter

    Available since

    Sept. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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