We narrowed to 26,935 results for: STI

1. pLenti Dest BlastR R1-R2

   Plasmid

   #84574

   Purpose

   Gateway destination vector expressing Blasticidin resistance under a PGK promoter

   Depositor

   Ghassan Mouneimne

   Article

   Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC034 - LwCas13a-msfGFP-2A-Blast

   Plasmid

   #91924

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCCM’

   Plasmid

   #162709

   Purpose

   pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   p15A origin replaced with high-copy colE1 origin

   Promoter

   PLtet0-1 promoter

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB’

   Plasmid

   #162703

   Purpose

   pCB reconstructed after selection for CCMB1 growth on glycerol under ambient air

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   pHnCB10 derived carboxysome operon, prk
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5513T>G (tetR E37A); 7758G>T (second tet op…

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDRM98 mKate2 Blast

   Plasmid

   #174720

   Purpose

   Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   mKate2
   BSD
   

   Use

   Lentiviral

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   MND and MND (off mKate-P2A)

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP1830

   Plasmid

   #70708

   Purpose

   Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Insert

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Mammalian

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   CAG

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVAX1-BMP2

   Plasmid

   #137909

   Purpose

   Constitutive mammalian overexpression vector for human bone morphogenetic protein 2 cDNA

   Depositor

   Georg Feichtinger

   Article

   Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

   Insert

   BMP2 (BMP2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDual-IN-SMN1

   Plasmid

   #215721

   Purpose

   dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN1 EXON 7 (SMN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

    Plasmid

    #205540

    Purpose

    Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    TRE3GS

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-6xHp

    Plasmid

    #214410

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    Spastin aa 43-92 (SPAST Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV7.1_3xFLAG-TAOK1

    Plasmid

    #172991

    Purpose

    constitutive expression of FLAG-tagged TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK1 (TAOK1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV7.1_3xFLAG-TAOK2

    Plasmid

    #172992

    Purpose

    constitutive expression of FLAG-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-TAOK2 (TAOK2 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. hCD12

    Plasmid

    #225541

    Purpose

    Expresses human histone deacetylase 6 (HDAC6) catalytic domain 1 and 2 (CD12), also known as hCD12, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Human Histone Deacetylase 6 Catalytic Domain 1 and 2 (HDAC6 Human)
    

    Use

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK2101

    Plasmid

    #65770

    Purpose

    Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized SaCas9, and SaCas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS-3xFlag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid

    #115136

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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