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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,006 results for: actin

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Showing: 3041 - 3060 of 6006 results

  6. pAAV.CAG.FLEX.iGluSnFR3.v857.GPI

    Plasmid
    #196218
    Purpose
    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI
    Depositor
    Kaspar Podgorski
    Publication
    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4
    Insert
    pAAV CAG.FLEX iGluSnFR3 v857.GPI
    Use
    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…
    Tags
    GPI Anchor, IgK-chain, and Myc epi tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    196218-plasmid-map-sequence-id-405482

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  8. AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

    Plasmid
    #199252
    Purpose
    Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    High specificity eSpCas9(1.1)-D10A nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A K1003A R1060A
    Promoter
    CAG promoter
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199252-plasmid-map-sequence-id-391383

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  10. pAK_DR30_CasRx_Puro

    Plasmid
    #134845
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A Puromycin
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    134845-plasmid-map-sequence-id-266706

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  14. PB-PEmax SpG

    Plasmid
    #187895
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, SpG variant
    Depositor
    Tobias Cantz
    Publication
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187895-plasmid-map-sequence-id-370756

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  15. pSLQ10715

    Plasmid
    #183960
    Purpose
    U6-crOct4-CAG-hyperdCas12a-miniVPR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    HyperdCas12a
    crRNA targeting Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183960-plasmid-map-sequence-id-363114

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  16. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Publication
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  19. pCAG-postASAP-p2a-ChrimsonR

    Plasmid
    #178794
    Purpose
    Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR
    Depositor
    Rafael Yuste
    Publication
    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FingR.PSD95, ASAP2f and ChrimsonR
    Tags
    mRuby2
    Expression
    Mammalian
    Mutation
    mutations in amino acids L146G, S147T N149R, S150…
    Promoter
    CAG
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178794-plasmid-map-sequence-id-350048

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Showing: 3041 - 3060 of 6006 results