We narrowed to 6,999 results for: nsf

1. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

   Plasmid

   #71219

   Purpose

   dCas9 fused to the human DNMT3B catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3B(E697A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER-retro-puro-Smad2

   Plasmid

   #89827

   Purpose

   Knockdown human Smad2 expression in human mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

   Insert

   SMAD2 shRNA (SMAD2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. His6-SMT3-SopB(C460S)

   Plasmid

   #183677

   Purpose

   Recombinant Salmonella Typhimurium SopB (catalytically inactive)

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   C460S

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS L30 Ruby3-Rab7A

   Plasmid

   #135651

   Purpose

   Rab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Tags

   Ruby3

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBL472

   Plasmid

   #126457

   Purpose

   Expresses Cas9-10xFlag-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   10x Flag
   TdT (DNTT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGal-Trans-Myc-HA-GFP11-KDEL

   Plasmid

   #177724

   Purpose

   ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
   

   Use

   Tags

   GFP11, HA, KDEL, and Myc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41s-GST-ATF6a-AD

   Plasmid

   #64774

   Purpose

   bacterial expression of GST-tagged ATF6 (1-150)

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   ATF6a-AD (1-150) (ATF6 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   C-term deletion

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5-Flag-Med25-VWA

   Plasmid

   #64772

   Purpose

   Mammalian expression of Flag-tagged Med25-VWA

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25-VWA (MED25 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C-terminal deletion

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-bio-myc-mCE 2-210

   Plasmid

   #112715

   Purpose

   Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   biotinylation signal peptide (Tagwerker et al. 20…

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV

   Available since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti HsATP13A2 P652_E653 del

    Plasmid

    #213700

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

    Depositor

    Veerle Baekelandt

    Article

    Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

    Insert

    human ATP13A2 P652_E653 del (ATP13A2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT, D962N

    Promoter

    CMV

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

    Plasmid

    #207135

    Purpose

    DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG-GW1-hCIT

    Plasmid

    #203166

    Purpose

    Yeast expression vector for human DHDDS

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    DHDDS (DHDDS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW57.1-Gata6-3xT7

    Plasmid

    #200889

    Purpose

    Inducible lentiviral expression of Gata6

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Gata6 (Gata6 Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV10-3xFLAG-prs-hMIGA2 (170-593)

    Plasmid

    #192833

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. mitoTALECD for_atp1_1397NC

    Plasmid

    #186204

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1397N of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397C of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Tags

    Expression

    Mutation

    Promoter

    Arabidopsis RPS5A

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. mitoTALECD for_-20G_1333CN

    Plasmid

    #186200

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. mitoTALECD for_ -13G_1397CN

    Plasmid

    #186199

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mitoTALECD for_ -6G_1333CN

    Plasmid

    #186198

    Purpose

    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

    Inserts

    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    

    Use

    Synthetic Biology; Ti plasmid for plant transform…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid

    #182887

    Purpose

    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    silet mutations in aa 197 - 203 to confer resista…

    Promoter

    EF1alpha core

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti_EGFP-P2A_truncHaus6_IRES_Blast

    Plasmid

    #182885

    Purpose

    Transfer vector for production of lentivirus. Control plasmid for rescue experiment of HAUS6 depletion phenotype, containing a nonsense mutation and a truncation in HAUS6.

    Depositor

    Daniel Gerlich

    Article

    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168

    Insert

    HAUS 6 (HAUS6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    stop codon introduced 2 amino acids downstream of…

    Promoter

    EF1alpha core

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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