We narrowed to 9,350 results for: pho;

1. pEGFP-RepoMan-T394A

   Plasmid

   #204994

   Purpose

   Expresses shRNA resistant GFP-RepoMan T394A Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell devision cycle associated 2 (CDCA2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Threonine 394 to Alanin

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAdEasy-hALDOA D34S

   Plasmid

   #210813

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Adenoviral

   Tags

   HA

   Expression

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBoBi-hALDOA D34S

   Plasmid

   #210807

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-CEP55(160-217, S215C)

   Plasmid

   #186793

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CEP55(160-217, S215C) (CEP55 Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   S215C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB1-ΔN-CHMP4C(G154C)

   Plasmid

   #190783

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   G154C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB1-ΔN-CHMP4C(M165C)

   Plasmid

   #190784

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   M165C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1-ΔN-CHMP4C(S191C)

   Plasmid

   #180027

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   S191C

   Promoter

   Available since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a Sa-iPGM-bio

   Plasmid

   #179523

   Purpose

   Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   S. aureus iPGM bio-6His
   

   Use

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21a Mo-iPGM-bio

   Plasmid

   #179614

   Purpose

   Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   M. orale iPGM bio-6His
   

   Use

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a Hp-iPGM-bio

    Plasmid

    #179615

    Purpose

    Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    H. pylori iPGM bio-6His
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSmChnERT(#99)

    Plasmid

    #184063

    Purpose

    cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

    Insert

    mCherry-nls-ERT2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet1-LanCL2-C321A/C367A-C-Ter-His

    Plasmid

    #154188

    Purpose

    To express LanCL2-C321A/C367A in bacterial cells

    Depositor

    Wilfred van der Donk

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    LanCL2
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Cys321->Ala321; Cys367->Ala367

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX307_PDHA2S291D

    Plasmid

    #115193

    Purpose

    Lentiviral transduction and expression of PDHA2S291D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S291D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S291D

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307_PDHA2S293D

    Plasmid

    #115194

    Purpose

    Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S293D

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX307_PDHA2S291D/S293D

    Plasmid

    #115195

    Purpose

    Lentiviral transduction and expression of PDHA2S291D/S293D into any mammalian cell

    Depositor

    Mohan Babu

    Article

    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

    Insert

    PDHA2S291D/S293D (PDHA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    p.S291D/S293D

    Promoter

    EF1alpha

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. SYKA-c022

    Plasmid

    #175490

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

    Plasmid

    #166855

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K683E, H685E, K686E

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

    Plasmid

    #166854

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    F641E, I645E

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

    Plasmid

    #166853

    Purpose

    Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

    Insert

    Atg13[571–700] (ATG13 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    F641G, I645G

    Promoter

    pCu

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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