We narrowed to 17,037 results for: Plat

1. pCASP-SptP120-3m160-HilA

   Plasmid

   #153325

   Purpose

   Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-3m160 DARPin
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. KK402: pMVP/Zeo-DEST

   Plasmid

   #121859

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

   Plasmid

   #149590

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGK:HygroR-CMV:mKO2-C_UTR

   Plasmid

   #68481

   Purpose

   Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Inserts

   Hygromycin Resistance
   mKO2-C_UTR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   shRNA sensor cassette "B" added to 3&#x…

   Promoter

   CMV and pGK

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. KP201: pMVP (L5-L4) eGFP-P2A

   Plasmid

   #121708

   Purpose

   pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eGFP-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. KP301: pMVP (L5-L4) mCherry-P2A

   Plasmid

   #121710

   Purpose

   pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   mCherry-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HE401: pMVP (L1-R5) shRNA

   Plasmid

   #121698

   Purpose

   pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-GW-V5-Hygro

   Plasmid

   #68265

   Purpose

   Constitutive selectable expression of C-term V5 tagged proteins

   Depositor

   Mitchell Guttman

   Article

   McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

   Type

   Empty backbone

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCASP-SptP120-E3_5-HilA

   Plasmid

   #153326

   Purpose

   Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   SptP120-E3_5 DARPin
   

   Use

   Salmonella expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LF601: pMVP (L1-R5) TBG promoter

    Plasmid

    #121695

    Purpose

    pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TBG promoter (Liver specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCASP-SptP120-K27-HilA

    Plasmid

    #153328

    Purpose

    Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-K27 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLyGo-Ec-6

    Plasmid

    #163132

    Purpose

    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9S(RBSmut)_Psyn-sgRNAmreB

    Plasmid

    #149594

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi mreB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAmreB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. B. fragilis mRNA toehold switch sensor

    Plasmid

    #110706

    Purpose

    Toehold switch sensor to detect a species specific mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    B. fragilis species specific toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. C. difficile toxin B mRNA toehold switch sensor

    Plasmid

    #110715

    Purpose

    Toehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    C. difficile toxin B toehold switch sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEC626

    Plasmid

    #174367

    Purpose

    Activation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.

    Depositor

    James Chappell

    Article

    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

    Insert

    Alpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23106

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLyGo-Kp-2

    Plasmid

    #163144

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p15aC-3D1D-5

    Plasmid

    #121050

    Purpose

    Bacterial expression of PIS, PI4K and PI3P5K mutant E545K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 3-kinase catalytic subunit p110α of class IA E545K
    

    Use

    Tags

    myc and none

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Tags

    Expression

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LC402: pMVP (L3-L2) eGFP + polyA

    Plasmid

    #121763

    Purpose

    pMVP L3-L2 entry plasmid, contains eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term eGFP fusion to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge