We narrowed to 37,743 results for: Ran;

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pYPQ233 (dLbCpf1-SRDX)

Plasmid

#86211

Purpose

Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

Insert

LbCpf1

Use

CRISPR

Tags

5' and 3' NLS and SRDX repressor

Expression

Plant

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes1+2 f.l. WT

Plasmid

#158540

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pmGFP-P2A-K(AAG)20-P2A-RFP

Plasmid

#105689

Purpose

Double fluorescent stalling reporter K(AAG)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLgw RFC1-V5-EcoDam

Plasmid

#59205

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_NoMTS_pET28

Plasmid

#34705

Depositor

David Chan

Article

Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Encodes residues 43–246, corresponding to full-le…

Promoter

T7

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer2

Plasmid

#14764

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-CAR-GECO1

Plasmid

#45493

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

CAR-GECO1

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-YAPS94A

Plasmid

#84010

Purpose

expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

Depositor

Stefano Piccolo

Article

Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

Insert

YAP1 (siRNA insensitive) (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

S94A

Promoter

TRE-CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-HDAC4 3SA

Plasmid

#45637

Depositor

Tso-Pang Yao

Article

Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

Insert

HDAC4 (HDAC4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

3SA

Promoter

CMV

Available Since

June 25, 2013

Availability

Academic Institutions and Nonprofits only

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pMD.RVG.CVS24-B2c

Plasmid

#19713

Depositor

Manfred Schubert

Article

Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

Insert

Rabies virus CVS24-B2c glycoprotein

Expression

Mammalian

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCMU-PMr

Plasmid

#61180

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pSVK3-Flag-MAN1

Plasmid

#26002

Depositor

Howard Worman

Article

Wu et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1361-7

Insert

LEMD3 LEM domain containing 3 (LEMD3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
mRNA-CleanCap entry vector

Plasmid

#188249

Purpose

Cloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A Human, (*see below))
humanized birA

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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hfCas12Max

Plasmid

#195336

Purpose

vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

human codon-optimized hfCas12Max

Tags

3xFlag

Expression

Mammalian

Mutation

N243R, E336R, D892R

Promoter

CBh, CMV, hU6

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pmGFP11C-Tau

Plasmid

#71434

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Tags

GFP (213-228)

Expression

Mammalian

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only