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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,082 results for: SON

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Showing: 3061 - 3080 of 31082 results

  4. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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  5. pCB-2

    Plasmid
    #210388
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available Since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210388-plasmid-map-sequence-id-418474

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  7. dCas9-dMSK1

    Plasmid
    #165602
    Purpose
    Expresses Sp dCas9 fused to truncated human MSK1 (42-802)
    Depositor
    Isaac Hilton
    Article
    Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1
    Insert
    S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 Human, Synthetic, S. Pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    165602-plasmid-map-sequence-id-327024

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  12. pAAV-phSyn-flex-SypEGFP

    Plasmid
    #153203
    Purpose
    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
    Depositor
    Lynette Lim
    Insert
    synaptophysin-EGFP
    Use
    AAV
    Promoter
    rat synapsin
    Available Since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153203-plasmid-map-sequence-id-298916

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  13. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  16. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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  18. pHN764

    Plasmid
    #178819
    Purpose
    Expression of EndoH in E. coli
    Depositor
    Kazuhiro Abe
    Insert
    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    Tags
    HIs8 and MBP
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178819-plasmid-map-sequence-id-351934

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  20. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Available Since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

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Showing: 3061 - 3080 of 31082 results