We narrowed to 17,501 results for: puro

1. HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

   Plasmid

   #25948

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 405 to Alanin…

   Promoter

   Available since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-C-Myc-DDK-IRES-Puro HNRNPR

   Plasmid

   #200730

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPR

   Depositor

   Claudio Alarcón

   Insert

   HNRNPR (HNRNPR Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-mir451 EF1Alpha-puro-T2A-BFP

   Plasmid

   #164792

   Purpose

   Express miR-451 under the U6 promoter in mammalian cells

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   microRNA 451a (MIR451A Human)
   puro-T2A-BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

   Plasmid

   #138524

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   PRC2-binding G-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

   Plasmid

   #177434

   Purpose

   To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

   Depositor

   David Andrews

   Insert

   Cb5 tail anchor (Cyb5r4 Rat)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

   Plasmid

   #72258

   Purpose

   Express FLPe, copGFP and Puromycin resistance gene under CB promoter

   Depositor

   Kazuhiro Oka

   Insert

   FLPe-P2A-copGFp-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CB and chicken beta-actin + CMV enhancer

   Available since

   Jan. 15, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

   Plasmid

   #22018

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Rac1 starts at I4 and contains mutation T17N.

   Promoter

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP

   Plasmid

   #75126

   Purpose

   Retroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   KMT6 (EZH2 Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mutation

   SET domain replaced with the SET domain of EZH1

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLenti CMV Puro DEST (w118-1) FAM149A

   Plasmid

   #107510

   Purpose

   Expressing FAM149a, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   FAM149A (FAM149A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

    Plasmid

    #209084

    Purpose

    Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    hSyn

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid

    #75120

    Purpose

    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Bromodomain replaced with the first bromodomain o…

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

13. XLone-Puro Cas13d P2A eGFP-NLS

    Plasmid

    #155182

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    CasRx
    

    Use

    Unspecified; Piggybac transposon

    Tags

    NLS and P2A eGFP NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-BRD9_N216A-PGK-Puro-IRES-GFP

    Plasmid

    #75116

    Purpose

    Retroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutation

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    BRD9 (BRD9 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    single amino acid mutation N216A

    Promoter

    Available since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

    Plasmid

    #64314

    Purpose

    chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

    Depositor

    Noriyuki Tsumaki

    Article

    Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

    Inserts

    COL11A2 promoter (COL11A2 Human)
    COL11A2 enhancer (COL11A2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and IRES-PURO

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid

    #171995

    Purpose

    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV hUbC-Cas9-P2A-Puro_NFATC2-sgRNA

    Plasmid

    #188704

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human NFATC2

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Insert

    NFATC2 sgRNAs
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

    Plasmid

    #22032

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mPA-GFP-PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. 1130 pBabe puroL HA FKHR H215R AAA

    Plasmid

    #9035

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

    Plasmid

    #184380

    Purpose

    The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Myod1 (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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