We narrowed to 20,614 results for: ACE

Showing: 6201 - 6240 of 20614 results

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CRY2-mCherry-iTrkA

Plasmid

#106168

Purpose

mammalian expression of CRY2PHR (aa 1-498) fused to mCherry and intracellular domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

CRY2PHR mCherry iTrkA (CRY2 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

Plasmid

#200634

Purpose

Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

PTEN (PTEN Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MTK5_012

Plasmid

#123856

Purpose

Encodes ConRE' and the human A1 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-A1 Insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-LacZa-T7

Plasmid

#173224

Purpose

pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-SF3B1-NTerm

Plasmid

#97425

Purpose

Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

Depositor

Alex Minella

Article

Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

Insert

SF3B1-N-term (AA1-500)

Tags

GST

Expression

Bacterial

Mutation

Silent mutation at nt876, aa289 (AGG to AGA)

Promoter

tac

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK403

Plasmid

#110599

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAV11K

Plasmid

#63184

Purpose

pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3_008

Plasmid

#123723

Purpose

Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS-VP16-EL222

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPKm-102

Plasmid

#90493

Purpose

pcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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MTK0_030

Plasmid

#123969

Purpose

Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phiC31-hygro-mRuby2PEST landing

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1-N1011

Plasmid

#92320

Purpose

N-term 1-1011 of HCF-1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

Insert

HCF-1 (HCFC1 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 2-1011

Promoter

CMV

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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MTK7_013

Plasmid

#123880

Purpose

Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_004

Plasmid

#123699

Purpose

Encodes mouse pPGK1 as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pPGK1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAV114

Plasmid

#63181

Purpose

pRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_FLAG_Mx PylRS

Plasmid

#140013

Purpose

Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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R0010 (pLacI)_AB

Plasmid

#66004

Purpose

MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK614

Plasmid

#110608

Purpose

BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

dCas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-Lyn11

Plasmid

#202683

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

LYN(1-11):PAmCherry1 (LYN Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEasyG3_hph

Plasmid

#184919

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-Squ:mCherry

Plasmid

#20163

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

Plasmid

#127322

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK401

Plasmid

#110597

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
MTK4a_019

Plasmid

#123832

Purpose

Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d2PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

Plasmid

#140014

Purpose

Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Mutation

Y126A, Y197F

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_014

Plasmid

#123929

Purpose

Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBIattB destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV DCC mut

Plasmid

#16460

Depositor

Bert Vogelstein

Article

Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

Insert

DCC (DCC Human)

Expression

Mammalian

Mutation

5' end of DCC

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pBTK601

Plasmid

#110607

Purpose

BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Cas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPKm-196

Plasmid

#90511

Purpose

pcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PIF6-DBD

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

Plasmid

#140201

Purpose

Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Cloning of crrna an…

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD102

Plasmid

#131403

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD102

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-synPCB2.0

Plasmid

#139477

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Expression

Mammalian

Mutation

V413L

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8b_005

Plasmid

#123893

Purpose

Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' AAV ITR

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_021

Plasmid

#123802

Purpose

Encodes VPR activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VPR AD

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NET30 pEGFP-N2 (815)

Plasmid

#62011

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET30 (MOSPD3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pBAD-HisD-mNG-C-Kbp

Plasmid

#178013

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-C-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6201 - 6240 of 20614 results

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