We narrowed to 8,345 results for: construct

1. pNH058

   Plasmid

   #42161

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   TAP Tag::mTFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCGN R-Ras 38V, CVLS

   Plasmid

   #14735

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   38V, CVLS

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pCGN R-Ras 38V, SVLL

   Plasmid

   #14736

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   38V, SVLL

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pCGN R-Ras 38V, SPCVLL

   Plasmid

   #14737

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   38V, SPCVLL

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pCKTRBS-LmrR-BzdB1_nSP

   Plasmid

   #102926

   Purpose

   pCKTRBS derivative carrying LmrR-BzdB1_nSP chimera, also known as ChBz02

   Depositor

   George Church

   Article

   Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

   Insert

   LmrR-BzdB1_nSP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtetO

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. rAAV-CAG::FLEX-rev:: hM4D-2a-GFP (AAV1)

   Viral Prep

   #52536-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from rAAV-CAG::FLEX-rev:: hM4D-2a-GFP (#52536). In addition to the viral particles, you will also receive purified rAAV-CAG::FLEX-rev:: hM4D-2a-GFP plasmid DNA. CAG driven, Cre-dependent expression of hM4D and GFP. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Scott Sternson

   Article

   Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

   Promoter

   CAG

   Tags

   GFP

   Available since

   Dec. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

7. MM39

   Bacterial Strain

   #42894

   Depositor

   Bryan Berger

   Article

   Su et al J Biol Chem. 2012 Jul 22.

   Bacterial Resistance

   Streptomycin

   Available since

   March 28, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

   Plasmid

   #185913

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRA1EGFP

    Plasmid

    #193791

    Purpose

    Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRK892

    Plasmid

    #71803

    Purpose

    Expression of mutated L. gasseri gusA from strong Ppgm promoter

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

    Insert

    gusA3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D573A

    Promoter

    Ppgm

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAM1

    Plasmid

    #60487

    Purpose

    This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Kras Wt

    Plasmid

    #75282

    Purpose

    Expresses human Kras wild-type

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None. Wild-Type

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCGN-SRF

    Plasmid

    #11977

    Purpose

    For mammalian expression of human SRF with an HA tag

    Depositor

    Ron Prywes

    Article

    Johansen et al Mol Cell Biol. 1993 Aug . 13(8):4640-7.

    Insert

    SRF (SRF Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMuLE_ENTR_12GLI-FLuc_R4-R3

    Plasmid

    #113712

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    12GLI-FLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCambia1300-PacI

    Plasmid

    #44183

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p306-BrdU-Inc

    Plasmid

    #71792

    Purpose

    BrdU-incorporating plasmid with selectable marker URA3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGolden-Neo

    Plasmid

    #51422

    Purpose

    Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK1 phosphoglycerate kinase 1 (PGK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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