We narrowed to 11,554 results for: Vars;

1. pMP4516

   Plasmid

   #107775

   Purpose

   Expression of ECFP

   Depositor

   Herman Spaink

   Article

   Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

   Insert

   pLacZ-ECFP in pBBR1-mcs5
   

   Use

   Tags

   pLacZ-ECFP

   Expression

   Bacterial

   Mutation

   Promoter

   pLacZ

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCS2+ Bmi1-cherry-HA

   Plasmid

   #30516

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   Bmi1 (Bmi1 Mouse)
   

   Use

   Xenopus/zebrafish expression

   Tags

   cherry and triple HA

   Expression

   Mammalian

   Mutation

   NO STOP

   Promoter

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mTFP1-CathespinB-6

   Plasmid

   #55467

   Purpose

   Localization: Lysosomes, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   CathespinB
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAcGFP1-N1-RvLEAM

   Plasmid

   #90035

   Purpose

   To express GFP-tagged RvLEAM in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   RvLEAM
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. YPRCΔ15α1

   Plasmid

   #101712

   Purpose

   GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZH519

   Plasmid

   #102672

   Purpose

   Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZH522

   Plasmid

   #102675

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CP1300-ABEmax

   Plasmid

   #119810

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YPRCΔ15Ω1

   Plasmid

   #101710

   Purpose

   GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV+ plasmid with onboard mCherry cassette

    Plasmid

    #169741

    Purpose

    CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAcGFP1-N1-CAHS3

    Plasmid

    #90031

    Purpose

    To express GFP-tagged CAHS3 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZH518

    Plasmid

    #102671

    Purpose

    Tetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-mSmo-WT-V5

    Plasmid

    #126415

    Purpose

    Localization, Expression of Smoothened WT in mammalian cell lines.

    Depositor

    Stacey Ogden

    Article

    Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

    Insert

    m-Smoothened (Smo Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCK671.306

    Plasmid

    #192641

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Plas-gRNA-LEU2

    Plasmid

    #157656

    Purpose

    GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    GFP-gRNA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBSc + MShab

    Plasmid

    #16146

    Depositor

    Larry Salkoff

    Article

    Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

    Insert

    Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pET-sCS4-2T

    Plasmid

    #28250

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-Scm3-H4 with 2 Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and Scm3. An…

    Promoter

    Available Since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Pinco-mNrp1dCy

    Plasmid

    #21936

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and His

    Expression

    Mutation

    deleted amino acids 884-923

    Promoter

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only