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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 18,716 results for: IRE

Showing: 7901 - 7950 of 18716 results

  6. pHIT353

    Plasmid
    #204506
    Purpose
    In vitro transcription of RNA probes for in situ hybridization of CeRep55 lncRNAs in nematode (after linearization with NotI and BglI)
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    CeRep55 tandem repeats from Y73B3A (C. elegans)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    double-T7
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204506-plasmid-map-sequence-id-403959

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  7. pHIT318

    Plasmid
    #204508
    Purpose
    rpl-21 promoter-driven C04F12.1 construct tagged with 2xHA for MosSCI integration at locus ttTi5605 on Chr II in nematode
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    rpl-21p::2xHA::C04F12.1 (C. elegans)
    Use
    Tags
    2xHA
    Expression
    Worm
    Mutation
    Promoter
    rpl-21 (C. elegans)
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    204508-plasmid-map-sequence-id-403957

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  8. pHIT246

    Plasmid
    #204507
    Purpose
    csr-1 construct tagged with 2xFLAG for MosSCI integration at locus ttTi5605 on Chr II in nematode
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    2xFLAG::csr-1 (C. elegans) (csr-1 Nematode)
    Use
    Tags
    2xFLAG
    Expression
    Worm
    Mutation
    Promoter
    csr-1 (C. elegans)
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    204507-plasmid-map-sequence-id-403950

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  9. pHIT334

    Plasmid
    #204516
    Purpose
    Bacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C-terminal fragment of coh-3 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204516-plasmid-map-sequence-id-403820

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  10. pHIT203

    Plasmid
    #204512
    Purpose
    Bacterial expression of C04F12.1 with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    C04F12.1 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204512-plasmid-map-sequence-id-403815

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  11. pHIT239

    Plasmid
    #204522
    Purpose
    In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    T7 (minimum seq.)
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204522-plasmid-map-sequence-id-403812

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  13. pHIT521

    Plasmid
    #204520
    Purpose
    Bacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of cec-5 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204520-plasmid-map-sequence-id-403749

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  14. pHIT342

    Plasmid
    #204514
    Purpose
    Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
    Depositor
    Yuji Kohara
    Article
    Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:
    Insert
    N-terminal fragment of syp-1 (C. elegans)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    204514-plasmid-map-sequence-id-403743

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  17. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

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  18. NK810

    Plasmid
    #196407
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196407-plasmid-map-sequence-id-385018

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  19. NK811

    Plasmid
    #196408
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196408-plasmid-map-sequence-id-384995

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  26. p5’UTR-ORF1

    Plasmid
    #190566
    Purpose
    Holding vector containing the contiguous 5’UTR and ORF1 regions of human L1 (L1.3) to facilitate subcloning of ORF1 mutants into the full-length L1 reporter pRTC2-puro (Cook et al., 2015).
    Depositor
    Anthony Furano
    Article
    Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112
    Insert
    Contiguous regions of the human L1 (L1.3) 5’UTR and ORF1
    Use
    For dna propagation only
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    190566-plasmid-map-sequence-id-393237

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  27. pACT2-μ4

    Plasmid
    #198176
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-4 μ4
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    April 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    198176-plasmid-map-sequence-id-387540

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Showing: 7901 - 7950 of 18716 results