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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSd_44

Plasmid

#59966

Purpose

constitutive expression of T7 sigma fragment

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7 sigma fragment

Expression

Bacterial

Mutation

N/A

Promoter

Pcon sigma

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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Anti-HCN2 [N71/37R] (Recombinant Antibody)

Antibody

#184178-rAb

Purpose

Anti-HCN2 (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Mouse and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

Dec. 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits
pBAD-iPASnFR-H

Plasmid

#225237

Purpose

Bacterial expression of iPASnFR-H

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-H

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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UAS::TVA

Plasmid

#164487

Purpose

Expression of TVA

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Insert

TVA (CD320 Chicken)

Expression

Mammalian

Promoter

UAS

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pTS35.41

Plasmid

#106263

Purpose

Polycistronic expression of luc, ekivR using P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

luc-P2A-ekivR

Use

Luciferase and Synthetic Biology

Promoter

Tet-On

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSd_1

Plasmid

#59947

Purpose

Splitposon in KanR ColE1 backbone

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

Splitposon w/ KanR, LacI & PTac

Expression

Bacterial

Mutation

N/A

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMULTIrec

Plasmid

#43959

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

pA

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb92

Plasmid

#159424

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb92

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8739

Plasmid

#126905

Purpose

Template for PCR amplification of XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtYB

Use

Synthetic Biology

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB8380

Plasmid

#126914

Purpose

Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtE

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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FsC_pET29b

Plasmid

#215405

Purpose

Expression construct for FsC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

FsC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-DEST

Plasmid

#134903

Purpose

Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Type

Empty backbone

Tags

3x-Flag

Expression

Insect

Promoter

polyhedron

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D1D2D3

Plasmid

#155215

Purpose

Expresses human MUC2 residues 21-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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phGf

Plasmid

#22516

Depositor

Christopher Buck

Article

Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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