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pDONR221_SLC22A2

Plasmid

#131964

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A2 (SLC22A2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pEM705 Gsq

Plasmid

#109356

Purpose

GsX chimera with Gαq C-terminus

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsq chimera

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMH4

Plasmid

#52529

Purpose

serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

2x FLAG-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1

Plasmid

#49784

Purpose

Express YOD1 with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Tags

Flag-tag

Mutation

WT

Available Since

Dec. 16, 2013

Availability

Academic Institutions and Nonprofits only
pHA-4EHP

Plasmid

#17344

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
CMVdel-dimericTomato-WASp(CRIB)

Plasmid

#191450

Purpose

Visualizing endogenous & active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WASp

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-BCL2

Plasmid

#163611

Purpose

Overexpression of human BCL2

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Insert

BCL2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pKSCTMF

Plasmid

#85073

Purpose

Gene insertion/deletion in Pichia pastoris

Depositor

Sheng Yang

Article

Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi

Insert

AOX1promoter - mazF - AOX1 tt- Zeocin R gene

Use

Gene insertion/deletion in pichia pastoris

Promoter

pAOX1

Available Since

March 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BaLgp120-8his

Plasmid

#100925

Purpose

Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence and Purification tag after K5…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pmiRA1-MYB

Plasmid

#141120

Purpose

Lentiviral overexpression of MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Chimeric_Butterfly_CY_1.0

Plasmid

#59800

Purpose

Genetically encoded fluorescent voltage indicator

Depositor

Thomas Knopfel

Article

Mishina et al Front Mol Neurosci. 2014 Sep 29;7:78. do

Insert

Cyan-Yellow Chimeric Butterfly Voltage Sensitive Fluorescent Protein

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLNC Wnt-3HA

Plasmid

#18029

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3 (WNT3 Human)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only
pMAL-c5X-ELP[V-60]

Plasmid

#67013

Purpose

Expresses malE-ELP[V-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V-60]

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMU-TGN41r

Plasmid

#61176

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-GR-GECO1.1

Plasmid

#42186

Depositor

Robert Campbell

Article

Hoi et al J Am Chem Soc. 2012 Dec 26.

Insert

GR-GECO1.1

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
DENV4 Rluc Reporter GND mutant Replicon

Plasmid

#118586

Purpose

For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.

Depositor

Radhakrishnan Padmanabhan

Article

Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.

Insert

DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.

Use

Yeast-e. col shuttle vector prs424

Mutation

The conserved GDD in the NS5 polymerase gene muta…

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Expression

Bacterial and Yeast

Promoter

REV1

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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5407 pClpB

Plasmid

#1235

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ClpB

Expression

Bacterial

Mutation

none

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only