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pGS-M1-BRG1-3xFLAG-BAF57-BAF60A

Plasmid

#109190

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1, untagged BAF57 and untagged BAF60A under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Inserts

BRG1 (SMARCA4 Human)
BAF57 (SMARCE1 Human)
BAF60A (SMARCD1 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Publication

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T2_gRNA

Plasmid

#197647

Purpose

pDEL160; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type II sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type II sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T1_gRNA

Plasmid

#199332

Purpose

pDEL159; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type I sgRNA

Depositor

William Talbot

Publication

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type I sgRNA

Use

Tol2 destination vector

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)

Plasmid

#107247

Purpose

beta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.

Depositor

Bryan Roth

Publication

Roth et al bioRxiv 251769

Insert

ARRB2

Use

AAV

Tags

MYC, PYLcs, and YFP

Promoter

hSYN

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

Plasmid

#48283

Depositor

Scott Gradia

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pPB UbC Arrestin-uTEV2-V5; UAS-mCherry

Plasmid

#173123

Purpose

Expresses uTEV2 and reporter receiver construct in DIPG

Depositor

Alice Ting

Publication

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

Arrestin-uTEV2-V5; UAS-mCherry

Expression

Mammalian

Promoter

UbC

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

Plasmid

#48308

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Publication

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Publication

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pRESTA-FLAG-CLIP-SNAP-StrepTag

Plasmid

#248181

Purpose

For the bacterial expression of FLAG-CLIP-SNAP-StrepTag fusion protein.

Depositor

Alison Twelvetrees

Publication

Smith et al Adv Sci (Weinh). 2026 Jul 21:e76672. doi

Insert

FLAG-CLIP-SNAP-StrepTag

Use

1218

Tags

HisTag, FLAG, CLIP and SNAP, StrepTag

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

March 6, 2026

Availability

Academic Institutions and Nonprofits only

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XZ024 CMV-TO-bArrestin2-MCP (FLP-IN)

Plasmid

#233379

Purpose

ARRB2-tdMCP with a short linker

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ARRB2-tdMCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Plasmid

#125231

Purpose

For generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirus

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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Nb24-His6 (b-arrestin1 nanobody)

Plasmid

#259692

Purpose

E. coli periplasmic expression of the active-state-specific beta-arrestin1 (arrestin2) nanobody Nb24 with a C-terminal His6 tag

Depositor

Laura Wingler

Publication

Elgeti et al J Biol Chem. 2026 Feb;302(2):111117. doi

Insert

Nb24-His6

Tags

His6

Expression

Bacterial

Promoter

plac

Available since

Aug. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LATeNT(slow)-Arrestin

Plasmid

#240984

Purpose

Expresses LATeNT*-Arrestin in mammalian cells; used with CCR6-NanoLuc or KORD-NanoLuc

Depositor

Alice Ting

Publication

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

LATeNT*-myc-Arrestin

Tags

myc (internal)

Expression

Mammalian

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Plasmid

#125235

Purpose

Expresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cells

Depositor

Alice Ting

Publication

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

Plasmid

#48284

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

Plasmid

#48313

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Sumo

Expression

Bacterial

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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