We narrowed to 77,030 results for: rest

1. pBabe_puro_DEST_Flag_SOX2

   Plasmid

   #45270

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   MCOPS3 (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

   Plasmid

   #87827

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Promoter

   CMV promoter, tetracycline operator

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. LoxP2-Mb2Tomato-2A (SO240)

   Plasmid

   #99614

   Purpose

   To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2Tomato
   

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSW002-Pc-TorT(sp)-mNeonGreen

   Plasmid

   #205021

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   TorT-mNeonGreen
   

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available Since

   Sept. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe_puro_DEST_Flag_FNDC3B

   Plasmid

   #45269

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   FNDC3B (FNDC3B Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5 FRT TO GFP MPS1 K-R

   Plasmid

   #59819

   Purpose

   Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

   Insert

   Mps1 (TTK Human)
   

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   KD, catalytically inactive, D664A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe_puro_DEST_Flag_GPR160

   Plasmid

   #45274

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   GPR160 (GPR160 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

9. lenti-Cas9-VQR-GFP_activity_reporter

   Plasmid

   #87156

   Purpose

   This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   GFP and sgRNA targeting GFP (NGA restricted)
   

   Use

   Lentiviral

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Promoter

    U6

    Available Since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pWzl_neo_DEST_flag_SOX2

    Plasmid

    #45309

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MCOPS3 (SOX2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

12. AAVS1 intron 1 gRNA 27

    Plasmid

    #132396

    Purpose

    Targets AAVS1 intron 1, gRNA: ACCCCACAGTGGGGCCACTA, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCSP1-mp53AS-6xMUT

    Plasmid

    #20904

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

    Insert

    p53AS (Trp53 Mouse)
    

    Expression

    Mammalian

    Mutation

    point mutation (Ser/Thr-Ala) of the 6 N-terminal …

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

14. PC1575

    Plasmid

    #232088

    Purpose

    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

    Depositor

    Pranam Chatterjee

    Article

    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

    Insert

    uAb with cloning Esp3I restriction site
    

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-sgTnr#2/Cre

    Plasmid

    #193245

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgTnr#2 (Tnr Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgTnr#1/Cre

    Plasmid

    #193244

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tnr gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgTnr#1 (Tnr Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pODN-H2BC11-TagBFP

    Plasmid

    #183867

    Purpose

    Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mTagBFP

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PEA1-Nuclease

    Plasmid

    #176528

    Purpose

    Delivers all prime editing nuclease components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1 intron 1 gRNA s3

    Plasmid

    #132395

    Purpose

    Targets AAVS1 intron 1, gRNA: GGGGCCACTAGGGACAGGAT, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid

    #82473

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

    Insert

    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Mouse, Synthetic)
    

    Tags

    TEV-Biotin and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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