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Showing: 451 - 480 of 17204 results

  9. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

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  10. Anti-Calretinin [L122/6-rat IgG2a] - Chimeric

    Plasmid
    #227043
    Purpose
    Mammalian expression plasmid of anti-Calretinin (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L122/6.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227043-plasmid-map-sequence-id-452416

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  11. Anti-Calretinin [L122/68-rat IgG2a] - Chimeric

    Plasmid
    #227044
    Purpose
    Mammalian expression plasmid of anti-Calretinin (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L122/68.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody. (CALB2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    227044-plasmid-map-sequence-id-449437

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  16. L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

    Plasmid
    #222875
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    MSCV LTR
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    222875-plasmid-map-sequence-id-441838

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  24. pET15b/EYFP-GgVcl 884-1066-ECFP FRET

    Plasmid
    #46179
    Depositor
    Susan Craig
    Insert
    Vcl 884-1066 (VCL Chicken)
    Use
    Tags
    ECFP, EYFP, and His
    Expression
    Bacterial
    Mutation
    aa 884-1066 (Vt, tail domain)
    Promoter
    T7
    Available since
    July 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    46179-plasmid-map-sequence-id-198213

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  26. α-catenin ECFP-YPET FRET ΔF-actin binding site

    Plasmid
    #198245
    Purpose
    Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
    Use
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted F-actin binding site on a-catenin (849-90…
    Promoter
    CMV
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    198245-plasmid-map-sequence-id-401145

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  28. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

    Plasmid
    #199567
    Purpose
    Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer
    Depositor
    Andrea Cimarelli, Yuxin Song
    Article
    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11
    Insert
    Trim69 isoform B (codon optimized) (TRIM69 Human)
    Use
    Retroviral; Allows for puromycin selection
    Tags
    Flag
    Expression
    Mutation
    codon optimized
    Promoter
    Available since
    May 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199567-plasmid-map-sequence-id-392223

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Showing: 451 - 480 of 17204 results