We narrowed to 830 results for: tg;

1. mDlx-SynTagMA_post-2A-mCerulean

   Plasmid

   #130899

   Purpose

   Synaptic Tag for Mapping Activity in GABAergic neurons

   Depositor

   Simon Wiegert

   Article

   Perez-Alvarez et al Nat Commun. 2020 May 18;11(1):2464. doi:

   Insert

   SynTagMA
   

   Use

   AAV

   Tags

   mCerulean

   Expression

   Mammalian

   Promoter

   mDlx

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET14b-dabAB2

   Plasmid

   #133013

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACTIN-SOX2-PGK Cre

   Plasmid

   #59019

   Purpose

   Bicistronic Lentivirus expressing sox2 and Cre

   Depositor

   Trudy Oliver

   Article

   Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Promoter

   B-actin

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV iGluu

   Plasmid

   #105832

   Purpose

   Fast indicator for extracellular glutamate imaging

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR S72T variant
   

   Tags

   Myc and PDGFR

   Expression

   Mammalian

   Mutation

   Changed Ser 72 to Thr

   Promoter

   CMV

   Available Since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LADL Anchor

   Plasmid

   #127664

   Purpose

   Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   dCas9-CIBN-2A-Puro
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 (D10A,H840A) mutant

   Promoter

   EF1a

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Human_TFAM_dHMGA_pET28

   Plasmid

   #167329

   Purpose

   Expresses TFAM dHMGA mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing linker, HMGB, and C-…

   Promoter

   T7

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

   Plasmid

   #52494

   Purpose

   Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

   Depositor

   Thomas Oertner

   Article

   Wietek et al Science. 2014 Mar 27.

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E90R, D156N, T159C

   Promoter

   human synapsin-1 and ribosomal skip sequence

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV CAG ChR2 E123T T159C 2A tDimer

   Plasmid

   #85399

   Purpose

   High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   CAG and ribosomal skip sequence 2A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFE-hCA

   Plasmid

   #132999

   Purpose

   pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Insert

   hCAII (CA2 Human)
   

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. bPAC-mycHis_pGEM

    Plasmid

    #85468

    Purpose

    Expression of photoactivated cyclase bPAC of Beggiatoa sp.

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    Bacterial Photoactivated Adenylyl Cyclase
    

    Tags

    His and Myc

    Promoter

    T7 promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn1 ChR2 ET-TC 2A tDimer

    Plasmid

    #101361

    Purpose

    High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Inserts

    Channelrhodopsin-2
    synaptophysin-red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E123T , T159C

    Promoter

    human Synapsin

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. NmeCas9_3XNLS_pMCSG7

    Plasmid

    #120078

    Purpose

    Bacterial expression of wtNmeCas9 with 3X NLS

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    NmeCas9
    

    Expression

    Bacterial

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV iGluf

    Plasmid

    #105833

    Purpose

    Fast indicator for extracellular glutamate imaging

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    CMV

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. RNAseq_cal_Tuschl

    Plasmid

    #59426

    Purpose

    Encodes sequence non-cognate to human genome for generation of RNAseq calibrators

    Depositor

    Thomas Tuschl

    Article

    Max et al Proc Natl Acad Sci U S A. 2018 Jun 5;115

    Insert

    RNAseq_cal_Tuschl
    

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-HE-Venus-bPAC(F198Y)

    Plasmid

    #165487

    Purpose

    Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Venus-bPAC(F198Y)-myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-His6-Ubc9-C93A

    Plasmid

    #133910

    Purpose

    Bacterial expression of His6-Ubc9-C93A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 93 to Alanine

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti_dNmeCas9

    Plasmid

    #120077

    Purpose

    dNmeCas9 with no sgRNA cassette lenti construct

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    dNmeCas9
    

    Use

    Lentiviral

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-His6-Ubc9-C138A

    Plasmid

    #133911

    Purpose

    Bacterial expression of His6-Ubc9-C138A

    Depositor

    Jeffrey Bode

    Article

    Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    Cysteine 138 to Alanine

    Promoter

    T7

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG-PIP70

    Plasmid

    #196808

    Purpose

    Contains GPAT5 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    GLYCEROL-3-PHOSPHATE SN-2- ACYLTRANSFERASE 5 (GPAT5 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGG-PIP50

    Plasmid

    #196788

    Purpose

    Contains ACS2 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    1-AMINO-CYCLOPROPANE-1-CARBOXYLATE SYNTHASE 2 (ACS2 Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Mutation

    150 C->G

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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