We narrowed to 59,421 results for: cloning

1. pDONR223 GABARAPL2 WT

   Plasmid

   #123086

   Purpose

   Gateway entry clone encoding human GABARAPL2 wild-type

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
   

   Use

   Gateway entry vector / entry clone

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-SMAD3

   Plasmid

   #79507

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   SMAD3 (SMAD3 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   FLAG

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-ReaChR-citrine

   Plasmid

   #169045

   Purpose

   Middle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insert

   Depositor

   Paola Arlotta

   Article

   Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

   Insert

   ReaChR-citrine
   

   Use

   Gateway entry cloning vector

   Tags

   citrine

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_C2813

   Plasmid

   #91092

   Purpose

   Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4 (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   PvUbi2

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. JH401: pMVP/Ad/RGD-PK-DEST

   Plasmid

   #121846

   Purpose

   pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 LC3A G120A

   Plasmid

   #123070

   Purpose

   Gateway entry clone encoding human MAP1LC3A G120A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Glycine 120 to Alanine

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD_B2517

   Plasmid

   #91074

   Purpose

   Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   TaU3

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-AKT-PH

   Plasmid

   #67674

   Purpose

   Gateway entry clone containing AKT-PH with no stop codon

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   AKT-PH
   

   Use

   Gateway multisite middle entry clone

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_C2806

   Plasmid

   #91091

   Purpose

   Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4 (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Promoter

   FMV 34S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223 LC3B G120A

    Plasmid

    #123073

    Purpose

    Gateway entry clone encoding human MAP1LC3B G120A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 120 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 GABARAPL2 G116A

    Plasmid

    #123087

    Purpose

    Gateway entry clone encoding human GABARAPL2 G116A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Glycine 116 to Alanine

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. IG301: pMVP (L1-R5) CMV promoter

    Plasmid

    #121692

    Purpose

    pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    CMV promoter
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. IF701: pMVP (L5-L4) APEX2

    Plasmid

    #121725

    Purpose

    pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 LC3C G126

    Plasmid

    #123079

    Purpose

    Gateway entry clone encoding human MAP1LC3C G126

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Deleted amino acids 127-147. Stop codon after G12…

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. KK001: pMVP/SB/Hygro-DEST

    Plasmid

    #121867

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IZ401: pMVP/Lenti/Neo-DEST

    Plasmid

    #121850

    Purpose

    pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Lentiviral; Pmvp gateway destination vector

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. KTK_136

    Plasmid

    #180520

    Purpose

    Empty backbone for D1.1 cloning, contains sfGFP dropout

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223 GABARAP no-stop

    Plasmid

    #123209

    Purpose

    Gateway entry clone encoding human GABARAP lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223 LC3C no-stop

    Plasmid

    #123208

    Purpose

    Gateway entry clone encoding human MAP1LC3C lacking stop codon, suitable for C-terminal tagging

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Stop codon removed

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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