We narrowed to 62,256 results for: cloning
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Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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Mucolipin1-pHcRed C1
Plasmid#62959Purposefull length human Mucolipin-1 cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
TagsHcRed1ExpressionMammalianMutationMucolipin-1 has 1 silent mutation compared to the…PromoterCMVAvailable SinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
dg263_pENTR_ChrimsonCel
Plasmid#66101PurposeEntry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertChrimson
UseGateway CloningAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRExpressionBacterialAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-nLuc
Plasmid#212611PurposeFor expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.DepositorInsertTornado-CVB3-nLuc
UseLuciferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-2xAM
Plasmid#92220PurposeLentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sitesDepositorInsertsSp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites
UseCRISPR and LentiviralTags2xAM tagExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterCBh and U6Available SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7RNAP
Plasmid#74096PurposeT7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNADepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterNoneAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-Puro-BsmBI_entry (pRW1484)
Plasmid#225752PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Puromycin resistance; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a URA3
Plasmid#61924PurposeExpression of S. cerevisiae URA3 cloned into pFA6aDepositorInsertURA3
UseYeast genomic targetingAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H2
Plasmid#79513PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-OC-IRES-BSD
Plasmid#53118PurposeTo create stable cell clones with high-level expression of Cas9 and OCT1DepositorInsertsUseLentiviralTagsIRES-BSD, NLS, and P2AExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGTMV
Plasmid#118755PurposeBinary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.DepositorInsertTobacco mosaic virus
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-NR1H3
Plasmid#79514PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDSDepositorInsertLXR Alpha (NR1H3 Human)
UseGateway CloningAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG2a_M18
Plasmid#105930PurposeExpression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.DepositorAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
bact2-mKate2-P2A-TurboID-dVHH[GFP]
Plasmid#163857PurposeZebrafish ubiquitous specific expression of mKate2-P2A-TurboID-dVHH[GFP]. Parton lab clone JBHDepositorInsertTurboID-VHH[EGFP]
UseZebrafish expressionTagsmKate2-P2A-TurboIDAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nuclear Pro-Code Vector Kit
Plasmid Kit#1000000197PurposeLentiviral plasmids with unique protein barcodes and gRNA cloning sites for CRISPR screens and tracking cell populations. Barcodes are linked to a nuclear-localizing mCherry fluorescent reporter. Part of the Pro-Code Vector Kits.DepositorApplicationBarcoding, Genome EditingVector TypeLentiviral, Mammalian ExpressionEditing TypeCRISPRAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a LEU2
Plasmid#61923PurposeExpression of K. lactis LEU2 cloned into pFA6aDepositorInsertLEU2
UseYeast genomic targetingAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
dg265_pENTR_CoChRCel
Plasmid#66103PurposeEntry clone containing CoChR coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2DepositorInsertCoChR
UseGateway CloningAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only