167,601 results

1. PB-PARP1-del.p119K120S–eGFP

   Plasmid

   #211554

   Purpose

   A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 119K 120S

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1-PARP1-delM43;F44I

   Plasmid

   #211579

   Purpose

   CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains deletion of M43 and F44I

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CHFR-PBZ-eGFP

   Plasmid

   #211581

   Purpose

   Expressing CHFR PBZ domain fused to eGFP

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   CHFR PBZ domain (CHFR Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   PBZ domain of the protein

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJR152

   Plasmid

   #196280

   Purpose

   Cassette used in dual sgRNA library generation

   Depositor

   Rebecca Voorhees

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Insert

   hU6-sgRNA-CR3
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJL049

   Plasmid

   #198809

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDR111_mannose_Bgal NLS

   Plasmid

   #188398

   Purpose

   Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Tags

   PhoD secretion peptide and SV40 NLS

   Expression

   Bacterial

   Promoter

   D-mannose

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-OSF-ATG13 (2-197)

   Plasmid

   #188075

   Purpose

   To make ATG101/ATG13 HORMA complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6HTC_CCDC124-Halo

   Plasmid

   #175328

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCDC124 (CCDC124 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCk4

   Plasmid

   #136698

   Purpose

   Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. RV-mCD4-HH5-4

    Plasmid

    #124984

    Purpose

    Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

    Depositor

    Dan Littman

    Article

    Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

    Insert

    HH5-4 TCRa P2A TCRb
    

    Use

    retroviral

    Expression

    Mammalian

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PDZK1_PDZ_2

    Plasmid

    #103943

    Purpose

    Protein expression and purification of PDZK1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PDZK1_PDZ_3

    Plasmid

    #103944

    Purpose

    Protein expression and purification of PDZK1 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    PDZK1 (PDZK1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    p10 and pH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-Mfn2

    Plasmid

    #141156

    Purpose

    Mammalian expression and labeling mitofusin 2

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Mitofusin 2 (MFN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1 gfaABC1D-cyto-GCaMP8s (AAV8)

    Viral Prep

    #186052-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pZac2.1 gfaABC1D-cyto-GCaMP8s (#186052). In addition to the viral particles, you will also receive purified pZac2.1 gfaABC1D-cyto-GCaMP8s plasmid DNA. gfaABC1D-driven expression of GCaMP8s, localized to the cystosol. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    GfaABC1D

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

16. pLYS1-FLAG-MitoGFP-HA

    Plasmid

    #50057

    Purpose

    Expression of GFP targeted to mitochondria with Flag and HA tags

    Depositor

    Vamsi Mootha

    Article

    Sancak et al Science. 2013 Nov 14.

    Insert

    Mito-GFP
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHcRed-NPM1wt-C1

    Plasmid

    #131818

    Purpose

    Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER21-puro_Gateway-3xFLAG

    Plasmid

    #172981

    Purpose

    Gateway Destination vector for doxycycline-inducible expression of ORF

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

    Plasmid

    #71485

    Purpose

    Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Type

    Empty backbone

    Promoter

    U6

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pgk-ATG-frt

    Plasmid

    #20734

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    pgk-ATG-frt-SAdpA-tetO
    

    Use

    Mouse Targeting

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. C-Terminal Split Cas9 with GyrA intein

    Plasmid

    #58694

    Purpose

    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

    Depositor

    Gang Bao

    Article

    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

    Insert

    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    

    Use

    AAV and CRISPR

    Tags

    GyrA Csplit Intein and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-84

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pAAV-Ef1a-Con/Foff 2.0-BFP (AAV8)

    Viral Prep

    #137130-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-BFP (#137130). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-BFP plasmid DNA. EF1a-driven, Cre-dependent expression of BFP (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Tags

    BFP (Cre-dependent)

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

26. lenti-EF1a-dCas9-KRAB-Puro

    Plasmid

    #99372

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-KRAB-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. LBR-mCherry

    Plasmid

    #172446

    Purpose

    Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    lamin B receptor (LBR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Tag5Amyc-GSK3b CA

    Plasmid

    #16261

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    GSK3 beta (GSK3B Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Constitutively active: S9A

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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29. pTlpA39-Wasabi

    Plasmid

    #86116

    Purpose

    mWasabi reporter gene gated by TlpA39

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA39
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A, D135V, A217V, L236F

    Promoter

    pTlpA and pTlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pHAGE-3FLAG-EEA1

    Plasmid

    #176491

    Purpose

    For lentiviral transduction of 3FLAG-EEA1

    Depositor

    Wade Harper

    Article

    Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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