We narrowed to 13,845 results for: Cas9

1. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

   Plasmid

   #107301

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
   

   Use

   CRISPR

   Tags

   FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Plasmid

   #101201

   Purpose

   Bacterial expression plasmid for SpCas9, REC3 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S701C/S960C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S701C and S960C

   Promoter

   T7

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Plasmid

   #101200

   Purpose

   Bacterial expression plasmid for SpCas9, REC2 FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C and D273C

   Promoter

   T7

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A

   Plasmid

   #101181

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1Q695+Q926A(N692A/M694A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A

   Plasmid

   #101182

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1M694+Q926A(N692A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A

   Plasmid

   #101183

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1N692+Q926A(M694A/Q695A/H698A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   M694A/Q695A/H698A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A

   Plasmid

   #101180

   Purpose

   Human expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-Cluster1H698+Q926A(N692A/M694A/Q695A/Q926A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N692A/M694A/Q695A/Q926A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

   Plasmid

   #69233

   Purpose

   R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

   Expression

   Mammalian

   Mutation

   Arginine 1335 is changed to Lysine

   Promoter

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALE_TS3

   Plasmid

   #69231

   Purpose

   Expresses R1335K mutant SpCas9 fused to TALE-TS3 in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS3 TALE
   

   Use

   CRISPR

   Tags

   2X NLS and NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

    Plasmid

    #187334

    Purpose

    dCas9 effector fusion for manipulating transcription

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid

    #188902

    Purpose

    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-KRAB(Kox1)-MeCP2
    

    Use

    Lentiviral

    Tags

    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid

    #190790

    Purpose

    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Inserts

    Cas9
    mazF-LVA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    silent mutations of BpiI-sites in original Cas9 g…

    Promoter

    PnrsB and Ptrc-[E*](104)

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

    Plasmid

    #85716

    Purpose

    Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Inserts

    Nme dCas9
    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO and U6

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188774

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and U6

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

    Plasmid

    #125430

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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