We narrowed to 66,514 results for: des.1
-
Plasmid#158759PurposeCell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseAAVExpressionMammalianPromotersynapsin promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV PHP.eB)
Viral Prep#20298-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsEYFP (Cre-dependent)Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-GCaMP6F (AAV5)
Viral Prep#137122-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB302
Plasmid#113673Purposeexpresses Cth SUMO protease in Escherichia coliDepositorInsertCth SUMO protease
Tags10-HisExpressionBacterialMutationdeleted amino acids 1-934PromoterT7Available SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDNA2.0 6H-TEV-KRAS
Plasmid#111849PurposeExpresses wild type human KRAS (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.DepositorInsertKRAS (KRAS Human)
Tags6xHis-tag and TEV protease cleavage sequenceExpressionBacterialPromoterT5Available SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIP-mEGFP-P2A-paAIP2/pAAV
Plasmid#91718PurposeAAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKIIDepositorInsertmEGFP-P2A-paAIP2
UseAAVPromoterCaMKII 1.3Available SinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Synapsin-pHoenix-WPRE
Plasmid#70111Purposelentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesiclesDepositorInsertpHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
UseLentiviralTagsArch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…PromoterSynapsinAvailable SinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
NLS-dCas9-trCIB1
Plasmid#64119PurposePhotoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1DepositorInsertdCas9-trCIB1 fusion
TagsHA and NLSExpressionMammalianMutationdCas9 (D10A H840A, catalytically inactive), trCIB…PromoterCMVAvailable SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
TagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpySrtA
Plasmid#51139Purposeexpression plasmid for N-terminal his tagged S.pyogenes SrtADepositorInsertS.pyogenes SrtA
Tags6x HisExpressionBacterialMutationDeleted amino acids 1-80PromoterT7Available SinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-LOV-VP16
Plasmid#42499PurposeLOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factorDepositorInsertFKF1 (N-LOV, G128D mutant) fused with VP16
TagsF-type VP16 activation domain and HA tagExpressionMammalianMutationG128D 1-168aaPromoterCMVAvailable SinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pri-let-7a-1
Plasmid#51377DepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-gfpmut3
Plasmid#65033PurposeTpR mini-Tn7 vector, mobilizable, for GFP tagging bacteriaDepositorInsertgfpmut3
ExpressionBacterialAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
mH2A1.1-CT-MYC
Plasmid#45166DepositorAvailable SinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-BroccoliSamSensor#1
Plasmid#124363PurposeOverexpression of circular RNA (Broccoli-based SAM sensor)DepositorInsertTornado-BroccoliSamSensor#1
ExpressionMammalianPromoterU6+27Available SinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianPromoterEF1AAvailable SinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL0283 (pTnsABC)
Plasmid#130633PurposeExpresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.DepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-NU
Plasmid#121139PurposeMammalian expression of GFP tagged CD274DepositorAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only