We narrowed to 16,201 results for: genome
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Plasmid#51710Purposecontains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.DepositorTypeEmpty backboneUseT. brucei genome integration vectorsTagsPTP (ProtC-TEV-ProtA)Available SinceMarch 12, 2014AvailabilityAcademic Institutions and Nonprofits only
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pICSL50005
Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyMutationG65S, L68V, A72S, Y203T, L231HAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458_CREB1_2
Plasmid#64940PurposeExpresses gRNA against human CREB1 along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-Blast-mU6
Plasmid#206806PurposepLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromotermU6Available SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET6
Plasmid#107438PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hulk-ubi-lacZ-to-Green
Plasmid#108641Purposeubiquitous promoter followed by a lacZ to GFP lineage tracing line. Cryst:venusGFPDepositorInsertubi-lacZ-to-GFP
Available SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYZ714
Plasmid#132955PurposecrRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombeDepositorInsertdFnCas12a
UseCRISPR and Synthetic BiologyTagsSV40 NLS and nucleoplasmin NLSPromoteradh1Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonorTp
Plasmid#211788PurposepDonor_ShCAST_kanR with modification of resistance cassette from KmR to TpRDepositorInsertdhfr
Available SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n-A71
Plasmid#125644Purposemicro-injection of poly-adenylated nCas9n RNADepositorInsertnCas9n
UseRna in vitro transcription vectorPromoterT3Available SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP Q157TAG
Plasmid#68297PurposeQ157TAG GFP reporterDepositorInsertGFP Q157TAG
TagsHisExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA43-GG
Plasmid#208014Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
Promoter14GAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p221z-AtMIR390a
Plasmid#118388PurposeEntry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertAtMIR390a backbone
UseRNAiAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pECNUS_MP01
Plasmid#194350PurposeCRISPR-Cas9 multiplexing acceptor clone with BpiI cutterDepositorInsertpcoCas9, FAST-Red
ExpressionPlantPromoterRPS5AAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH75
Plasmid#128224Purposeclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)DepositorInsertclassic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
peft-3::cas-9::tbb-2 3'UTR
Plasmid#48960Purposethis plasmid contains the codon optimized gene encoding Cas9 for C. elegans.DepositorInsertcas9
UseCRISPRPromotereef-1A.1 (eft-3)Available SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomSua1
Plasmid#107446PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertSua1
TagsV5-HisExpressionYeastMutationSee depositor comments below.PromoterGAL1Available SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-pomMet1
Plasmid#107444PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMet1
TagsV5-HisExpressionYeastPromoterGAL1Available SinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
BSP606
Plasmid#122251Purposeexpression vector for mECitrine under EFT3 promoterDepositorInsertmECitrine
ExpressionWormPromoterEFT3Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only