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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,496 results for: EMB

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Showing: 3241 - 3260 of 17496 results

  2. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105788-plasmid-map-sequence-id-203379

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  3. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pKK-mCherry-TEV

    Plasmid
    #105779
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mCherry-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105779-plasmid-map-sequence-id-203368

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  8. pKK-mRuby3-TEV

    Plasmid
    #105780
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mRuby3-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105780-plasmid-map-sequence-id-203369

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  9. pKK-TEV-mRuby3

    Plasmid
    #105797
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mRuby3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105797-plasmid-map-sequence-id-203388

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  10. pKK-TEV-MBP

    Plasmid
    #105787
    Purpose
    Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-MBP
    Expression
    Mammalian
    Available Since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105787-plasmid-map-sequence-id-211379

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  11. pKK-TEV-mCherry

    Plasmid
    #105796
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mCherry
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105796-plasmid-map-sequence-id-203387

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  14. pKK-EGFP-TEV

    Plasmid
    #105776
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    EGFP-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105776-plasmid-map-sequence-id-203365

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  15. USPL1cat-C236S-pEYFP_C1

    Plasmid
    #85767
    Purpose
    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S
    Depositor
    Frauke Melchior
    Article
    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10
    Insert
    USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    catalytic domain (aa 212-514), catalytic inactiv…
    Promoter
    CMV
    Available Since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85767-plasmid-map-sequence-id-169512

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  17. pKK-mTagBFP-TEV

    Plasmid
    #105772
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mTagBFP-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105772-plasmid-map-sequence-id-203361

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  20. pKK-TEV-mTagBFP

    Plasmid
    #105789
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mTagBFP
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105789-plasmid-map-sequence-id-203380

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Showing: 3241 - 3260 of 17496 results