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We narrowed to 79,168 results for: rest

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Showing: 3241 - 3260 of 79168 results

  1. pSW002-Pc-TorT(sp)-mNeonGreen

    Plasmid
    #205021
    Purpose
    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorT signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm
    Depositor
    Rosemarie Wilton
    Article
    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:
    Insert
    TorT-mNeonGreen
    Expression
    Bacterial
    Mutation
    mNeonGreen is codon optimized for expression in P…
    Promoter
    Pc
    Available Since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205021-plasmid-map-sequence-id-407999

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  6. RCAS-sgATG7

    Plasmid
    #75228
    Purpose
    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
    Depositor
    Noor Gammoh
    Article
    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:
    Insert
    Autophagy Related 7 (Atg7 Mouse)
    Use
    Retroviral
    Promoter
    U6
    Available Since
    June 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pODN-H2BC11-TagBFP

    Plasmid
    #183867
    Purpose
    Repair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    H2BC11 homology arms with linker-mTagBFP (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mTagBFP
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183867-plasmid-map-sequence-id-362447

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  14. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available Since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  15. ST*-GFP

    Plasmid
    #163667
    Purpose
    Expresses partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163667-plasmid-map-sequence-id-322800

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  18. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid
    #50866
    Purpose
    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C I730C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    50866-plasmid-map-sequence-id-76339

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  19. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid
    #50875
    Purpose
    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50875-plasmid-map-sequence-id-76348

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  20. pcDNA3.1 Flag YFP hNKCC1 N680C A734C (NT816)

    Plasmid
    #50870
    Purpose
    Expresses human NKCC1 N680C A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    N680C A734C in hNKCC1 in NT17
    Promoter
    CMV
    Available Since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50870-plasmid-map-sequence-id-76343

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Showing: 3241 - 3260 of 79168 results